Invitrogen™

Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry

Catalog number: A35135
Invitrogen™

Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry

Catalog number: A35135
Catalog Number
A35135
also known as A-35135
Unit Size
1 kit
Price (USD)
Price: 381.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A35135
also known as A-35135
1 kit
Price: 381.00
Your Price:
-
Product Overview
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The Vybrant™ DyeCycle™ Violet/SYTOX™ AADvanced™ apoptosis kit is used for the identification of apoptotic cells. This kit provides a rapid and convenient assay based upon fluorescence analysis of the compacted state of the nuclear chromatin in apoptotic cells. The kit contains solutions of the cell-permeant Vybrant™ DyeCycle™ Violet stain (Ex/Em max ∼370/440nm) which is excited with UV or violet laser and SYTOX™ AADvanced™ (Ex/Em max ∼546/650 nm) which is excited with a 488 nm laser. SYTOX™ AADvanced™ is used to detect changes in permeability of the plasma membrane therefore allowing identification of dead cells. The staining pattern resulting from simultaneous use of both dyes in combination make it possible to distinguish normal, apoptotic, and dead cell populations by flow cytometry.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Tube
Product Type
Apoptosis Kit
Flow Cytometer Laser Lines
405, 488
Excitation/Emission
SYTOX™ AADvanced™: 546⁄647, Vybrant™ DyeCycle™ Violet: 369⁄437
No. of Reactions
200
Shipping Condition
Room Temperature
Product Line
Vybrant™
Detection Method
Fluorescent
For Use With (Application)
Flow Cytometry
For Use With (Equipment)
Flow Cytometer
Label or Dye
Vybrant DyeCycle Violet, SYTOX AADvanced Dead Cell Stain
Label Type
Other Label(s) or Dye(s)

Contents & Storage

Contains 1 vial of Vybrant™ DyeCycle™ Violet (50 μL, 5mM solution in water); 1 vial SYTOX™ AADvanced™ stain (100 µg); and 1 vial of DMSO (200 μL).

Storage 2-6°C