Invitrogen™

Pacific Blue™ Annexin V/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry

Catalog number:  A35136

Related applications:

Flow Cytometry

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Description

This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to violet-fluorescent Pacific Blue™ dye and dead cells using SYTOX™ AADvanced™ stain. After staining a cell population with Pacific Blue™, annexin V and SYTOX™ AADvanced™, apoptotic cells show violet fluorescence, dead cells show red fluorescence, and live cells show little or no fluorescence. These populations are easily distinguished by a flow cytometer with the 405 nm and 488 nm lines for excitation. There is very little spectral overlap between the two dyes, therefore very little compensation is needed. Each kit contains sufficient reagents for approximately 50 flow cytometry tests.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission: Pacific Blue™: 415⁄455, SYTOX® AADvanced™: 546⁄647
Flow Cytometer Laser Lines: 405, 488
Label Type: Pacific Dyes
Label or Dye: Pacific Blue™, SYTOX® AADvanced™ Dead Cell Stain
No. of Reactions: 50
Shipping Condition: Wet Ice
Detection Method: Fluorescent
For Use With (Equipment): Flow Cytometer
Format: Tube(s)

Contents & storage

Contains 1 vial of annexin V, Pacific Blue™ conjugate (250 µL), 1 vial of SYTOX® AADvanced™ dead cell stain, and 1 bottle of annexin binding buffer (5X solution, 15 mL), and 1 vial of DMSO (100 µL).

Store in refrigerator (2–8°C) and protect from light.