Invitrogen™

Pacific Blue™ Annexin V/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry

Catalog number: A35136
Invitrogen™

Pacific Blue™ Annexin V/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry

Catalog number: A35136
Catalog Number
A35136
also known as A-35136
Unit Size
1 kit
Price (USD)
454.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A35136
also known as A-35136
1 kit
454.00
Your Price:
***
Product Overview
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This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to violet-fluorescent Pacific Blue™ dye and dead cells using SYTOX™ AADvanced™ stain. After staining a cell population with Pacific Blue™, annexin V and SYTOX™ AADvanced™, apoptotic cells show violet fluorescence, dead cells show red fluorescence, and live cells show little or no fluorescence. These populations are easily distinguished by a flow cytometer with the 405 nm and 488 nm lines for excitation. There is very little spectral overlap between the two dyes, therefore very little compensation is needed. Each kit contains sufficient reagents for approximately 50 flow cytometry tests.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission
Pacific Blue™: 415⁄455, SYTOX™ AADvanced™: 546⁄647
Flow Cytometer Laser Lines
405, 488
For Use With (Equipment)
Flow Cytometer
No. of Reactions
50
Shipping Condition
Wet Ice
Conjugate
Pacific Blue™, SYTOX™ AADvanced™ Dead Cell Stain
Format
Tube
Product Type
Apoptosis Kit

Contents & Storage

Contains 1 vial of annexin V, Pacific Blue™ conjugate (250 µL), 1 vial of SYTOX™ AADvanced™ dead cell stain, and 1 bottle of annexin binding buffer (5X solution, 15 mL), and 1 vial of DMSO (100 µL).

Store in refrigerator (2–8°C) and protect from light.

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