Invitrogen™
TrueGuide™ sgRNA Positive Control, HPRT1 (human)
Catalog number: A35524
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Description
Thermo Fisher Scientific provides a complete collection of validated positive and negative controls for the TrueGuide Synthetic gRNA product line to help maximize your editing performance. This positive control against HPRT1 targets an intronic region and has achieved editing efficiencies of up to 65% in a variety of human cell lines. Use the HPRT1 control to easily determine the optimal conditions for delivering CRISPR/Cas9 tools to your cell type, allowing you to move forward in your editing experiments with confidence and minimize downstream effort to isolate and validate your edited clones.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Unravel the complexities of cancer cells with robust CRISPR gene editing
Factors affecting genome editing efficiency outcomes
Optimized protocols provide minimal cell toxicity
Invitrogen CRISPR genome-editing tools outperform other suppliers
A CRISPR-Cas9 targeted double-strand break
Achieve functional knockout in human primary T-cells
Standard gene editing workflow using CRISPR-Cas9 technology
Engineered nuclease—various double strand repair pathways
CRISPR RNP—streamlined editing and minimization of off-target events
Robust CRISPR gene editing in THP-1 cells
Specifications
| Product Line: | TrueGuide™ |
|---|---|
| Product Size: | 3 nmol |
| Shipping Condition: | Room Temperature |
Contents & storage
Delivered as dried RNA. Store dried RNA at room temperature; store resuspended RNA at -5 to -30°C. Resuspend in nuclease-free water or TE.
