100% knock-in using CRISPR-Cas9—try our new TrueTag Donor DNA Kits for GFP, RFP or luciferase tagging.
Invitrogen™
TrueGuide™ sgRNA Positive Control, HPRT1 (human)
Description
Thermo Fisher Scientific provides a complete collection of validated positive and negative controls for the TrueGuide Synthetic gRNA product line to help maximize your editing performance. This positive control against HPRT1 targets an intronic region and has achieved editing efficiencies of up to 65% in a variety of human cell lines. Use the HPRT1 control to easily determine the optimal conditions for delivering CRISPR/Cas9 tools to your cell type, allowing you to move forward in your editing experiments with confidence and minimize downstream effort to isolate and validate your edited clones.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Engineered nuclease—various double strand repair pathways
Invitrogen CRISPR genome-editing tools outperform other suppliers
A CRISPR-Cas9 targeted double-strand break
Standard gene editing workflow using CRISPR-Cas9 technology
Achieve functional knockout in human primary T-cells
Optimized protocols provide minimal cell toxicity
CRISPR RNP—streamlined editing and minimization of off-target events
Factors affecting genome editing efficiency outcomes
Robust CRISPR gene editing in THP-1 cells
Unravel the complexities of cancer cells with robust CRISPR gene editing
Specifications
| Shipping Condition: | Room Temperature |
|---|---|
| Product Line: | TrueGuide™ |
Contents & storage
Delivered as dried RNA. Store dried RNA at room temperature; store resuspended RNA at -5 to -30°C. Resuspend in nuclease-free water or TE.
