Applied Biosystems™

VetMAX™ PRRSV EU & NA 2.0 Kit

Catalog number:  A35751

Related applications:

PCR & Real-Time PCR

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Description

The VetMAX PRRSV EU & NA 2.0 Kit is a ready-to-use, single-well real-time RT-PCR kit that enables reliable, rapid, and accurate detection of porcine reproductive and respiratory syndrome virus (PRRSV) strains in porcine samples. The European (EU) and North American (NA) strains are detected and differentiated using fluorescent hydrolysis probe chemistry. An exogenous internal positive control (IPC) is included.

The advanced technology of the VetMAX PRRSV EU & NA 2.0 Kit offers several benefits:
Highly accurate―updated design detects the four subtypes of the EU PRRSV Type 1 and NA PRRSV strains, including the HP-PRRSV strain
Fast―results obtained in less than two hours
Flexible―fast and standard amplification modes
Versatile―compatible with a variety of sample matrices, including serum, whole blood, semen, tissue, oral fluid, and cell culture supernatant samples in pools of up to five samples (serum, whole blood, and semen)

The kit offers the convenience of a simple and fast workflow combining the MagMAX CORE nucleic acid purification method with a fast PCR amplification mode for a result in less than two hours.

More than 780 field samples from 13 origins identified as positive or negative for PRRS virus by partner laboratories have been tested. The results showed 99.5% sensitivity and 99.6% specificity.

Regulatory requirements vary by country; products may not be available in your geographic area.

Specifications

For Use With (Equipment): 7500 System, QuantStudio™
Detection Method: Fluorescent
PCR Method: qPCR
Reaction Speed: Fast or Standard
Shipping Condition: Dry Ice
Product Line: VetMAX™

Contents & storage

The kit includes the following assays and reagents:
• 3 – Mix PRRS EU/NA 2.0: Contains primers, probes, buffer, and enzyme for optimized triplex real-time RT-PCR detection of PRRSV EU, PRRSV NA, and IPC targets
• 4a – EPC PRRS EU/NA 2.0: RNA template (already extracted) with PRRSV EU and PRRSV NA target sequences. It serves as an external positive control for the real-time RT-PCR components, and is used to set validation criteria for test results.
• 5 – IPC PRRS: Added to each test and control sample at the lysis step of the RNA isolation procedure. It serves as an exogenous internal positive control for the RNA isolation procedure, and is used to monitor for the presence of PCR inhibitors.

Store at -5 to -30°C.