PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit
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PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit
Invitrogen™

PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit

The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection자세히 알아보기
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카탈로그 번호반응 수
A3622750 Preps
A3589225 Preps
카탈로그 번호 A36227
제품 가격(KRW)
1,043,000
온라인 행사
Ends: 31-Dec-2025
1,158,000
할인액 115,000 (10%)
Each
카트에 추가하기
반응 수:
50 Preps
제품 가격(KRW)
1,043,000
온라인 행사
Ends: 31-Dec-2025
1,158,000
할인액 115,000 (10%)
Each
카트에 추가하기
The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (less than 1 EU/μg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.

NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold.

Advantages of the PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit include:
Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/μg endotoxins
High yields—isolate up to 0.4 mg of high-quality plasmid DNA
Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Cloning, PCR
반응 수50 Preps
프렙 스케일Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg
제품라인PureLink
제품 유형Plasmid Purification Kit
수량50 Preps
스케일Midi
배송 조건Room Temperature
테스트 시간30 min.
형식Kit
Isolation TechnologySilica Column
Unit SizeEach
구성 및 보관
• Resuspension buffer, store at 4°C upon reciept
• Lysis buffer, store at room temperature
• Precipitation buffer, store at room temperature
• Binding buffer, store at room temperature
• Wash buffer 1, store at room temperature
• Wash buffer 2 (concentrate), store at room temperature
• Elution buffer, store at room temperature
• Column assemblies, store at room temperature
• Syringe filters, store at room temperature
• Syringe plungers, store at room temperature
• Collection tubes, store at room temperature

자주 묻는 질문(FAQ)

Can I elute my plasmid from PureLink columns with water instead of TE?

For any silica columns, elution with water is generally possible. However, a buffer is preferred for stability and accuracy of absorbance readings, as pure water can have a very low pH (4 - 5).

What are endotoxins and why are their levels important for transfection?

Endotoxins are typically any cell-associated bacterial toxins that are part of the outer surface of the cell wall of gram-negative bacteria. Endotoxins can influence cell growth, cell differentiation, contractility, and protein expression in mammalian cells. Endotoxins are released during bacterial lysis, and they can subsequently reduce transfection efficiency and protein expression levels. Please review the following article for more information about endotoxins: Butash KA et al. (2000) Reexamination of the effect of endotoxin on cell proliferation and transfection efficiency. Biotechniques 29(3): 610-614, 616, 618-619.

What is the best way to determine the quality/purity of my plasmid DNA?

The ratio of absorbance at 260 nm to the absorbance at 280 nm (A260/A280) is typically used to measure purity of the sample. For DNA, the ideal A260/A280 ratio is 1.8, but it can be in the range of 1.7 - 1.9. The A260/A230 ratio is also used to determine if contamination is present. For DNA, the ideal A260/A230 ratio is between 1.8 and 2.0. DNA purity can also be examined by gel analysis. For plasmid DNA, look for a strong, single band (perhaps with a few extra bands representing multimers of the desired molecule). For genomic DNA, look for high average fragment sizes.

Do you offer a kit for plasmid extraction with endotoxin reduction?

We offer the following silica column-based plasmid purification kits for isolation of endotoxin-free or low-endotoxin plasmid DNA:
- PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit (Cat. No. A31217, A31231, A33073) (<0.1 EU/µg endotoxins):
https://www.thermofisher.com/order/catalog/product/A31217
- PureLink Fast Low-Endotoxin Midi (Cat. No. A35892, A36227) or Maxi (Cat. No. A36228, A35895) Plasmid Purification Kits (<1 EU/µg endotoxins):
https://www.thermofisher.com/order/catalog/product/A35892
https://www.thermofisher.com/order/catalog/product/A36228

Will kit performance be affected if I exceed culture input volumes with PureLink Fast Low-Endotoxin Plasmid Purification kits?

Our recommendation is to not exceed the maximum recommended culture volumes specified in the kit manuals; using larger culture volumes means increased cell mass, which will result in reduced plasmid yield and purity.