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Invitrogen™

TrueCut™ Cas9 Protein v2

Catalog number: A36499
Have Questions?
Invitrogen™

TrueCut™ Cas9 Protein v2

Catalog number: A36499
Have Questions?
Catalog Number
A36499
Unit Size
500 µg
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
100 µg
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
25 µg
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
10 µg
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A36499500 µg
-
A36498100 µg
-
A3649725 µg
-
A3649610 µg
-
Product Overview
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Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency. Features include:

• Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in difficult targets than the competition
• High quality—manufactured under strict ISO 13485 quality standards
• Validated protocols for a large number of cell types help you achieve success faster

TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.

• Transfection-ready, using either lipid-mediated transfection reagents or electroporation
• Eliminates time-consuming cloning steps
• Available in 2 concentrations:
    --1 μg/μL for use in standard editing scenarios
    --5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
    --For custom sizes or concentrations, please inquire

Options for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:
1. Order transfection-ready TrueGuide synthetic gRNAs
2. Generate transfection-ready gRNA in as little as four hours including template assembly with our GeneArt Precision gRNA Synthesis Kit
3. Have our Custom Services team design, synthesize, and purify in vitro (IVT) gRNA sequences for you

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
5 μg/μL
Components
100μL TrueCut Cas9 Protein v2 (5mg/mL)
Grade
Molecular Biology
Accession Number
NC_002737.2:854751-858857
Expression System
E. coli
Gene ID (Entrez)
901176
Gene Symbol
Spy_1046
Gene Alias
cas5, csn1
Protein Form
Recombinant
Protein Tag
Untagged
Shipping Condition
Dry Ice
Validated Application
Genome Editing
Product Type
Cas9 Protein v2
Research Category
Genome Editing
Product Line
Invitrogen™

Contents & Storage

100 μL TrueCut Cas9 Protein v2 (5 mg/mL)
Store at -5 to -30°C.

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