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B-27™ Plus Neuronal Culture System

Catalog number:  A3653401

Related applications:

Primary Cell Culture

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The B-27 Plus Neuronal Culture System is a next-generation media system that provides the highest rate of in vitro survival of primary rodent and human stem cell-derived neurons. Comprised of B-27 Plus Supplement (50X) and Neurobasal Plus Medium, this system is an evolution of the most cited neuronal cell culture products, B-27 Supplement and Neurobasal Medium. It features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.

The B-27 Plus Neuronal Culture System offers:
• At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Available as a 50X, 10 mL supplement with 500 mL basal medium, the B-27 Plus Neuronal Culture System can seamlessly replace other neuronal cell culture systems, such as classic B-27 Supplement and Neurobasal Medium. The individual components of the system are also available for purchase separately.

WARNING: Cancer –
For Research Use Only. Not for use in diagnostic procedures.


Classification: Serum-Free
Shipping Condition: Ambient
Product Line: B-27™, Neurobasal™
Cell Type: Neural Cells

Contents & storage

• 10 mL B-27 Plus Supplement (50X), store at –5 to –20°C, protect from light
• 500 mL Neurobasal Plus Medium, store at 2–8°C, protect from light