• 10 mL B-27 Plus Supplement (50X), store at –5 to –20°C, protect from light • 500 mL Neurobasal Plus Medium, store at 2–8°C, protect from light
よくあるご質問(FAQ)
Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?
We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.
I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?
We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.
How do I feed my cells with the B-27 Plus Neuronal Culture System?
We recommend performing half medium exchanges every 2-3 days post-plating. Make sure that neurons are not completely exposed to air by not removing all of the medium during exchanges.