The MagMAX DNA Multi-Sample Ultra 2.0 Kit is an automatable magnetic bead-based sample preparation technology for the isolation of genomic DNA (gDNA) from 50 µL to 2 mL of whole blood, saliva, buffy coat, and buccal swabs. The resulting purified gDNA is ideal for a variety of downstream molecular biology applications such as qPCR, next-generation sequencing, and microarray analysis. One kit is sufficient to process 200 50–200 µL samples or 100 200–400 µL samples.
Key features of the MagMAX DNA Multi-Sample Ultra 2.0 Kit: • Process 6–96 samples in 45 minutes with 5 minutes hands-on time • Isolate gDNA from 50 µL–2 mL of sample using a simple protocol in SBS microtiter plates • Skip sample and buffer volume normalization steps for hassle-free processing • Download validated BindIt software automation scripts for KingFisher Duo Prime and Flex purification systems • Automation-friendly stand-alone MagMAX DNA Multi-Sample Ultra 2.0 reagent bottle options available
Isolate genomic DNA from multiple sample types with a single sample prep kit The MagMAX DNA Multi-Sample Ultra 2.0 Kit isolates gDNA from 50 µL–2 mL of whole blood (figure below), fresh and stabilized saliva (figure below), and EDTA/Streck cfDNA tube buffy coat (figure below) samples. Additionally, the same kit can be used to isolate gDNA from buccal swabs and other biological sample types. The MagMAX DNA Multi-Sample Ultra 2.0 Kit maximizes gDNA yields by treating samples with proteinase K (PK) and PK-enhancer followed by an innovative lysis/binding solution to simultaneously lyse cells, denature proteins, and capture gDNA on the high-binding capacity Ultra 2.0 gDNA magnetic binding beads. The Ultra 2.0 Wash I solution removes proteins, detergents, and other impurities from the bound nucleic acid. The gDNA is then released from the beads using a low-TE elution solution.
Fast and hassle-free MagMAX magnetic bead-based gDNA workflows The MagMAX DNA Multi-Sample Ultra 2.0 Kit combines multiple laborious sample processing steps together into a single step to minimize the time and effort traditionally required by other methods without compromising yield and purity. Process a broad range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments for hassle-free sample prep. Simply match your sample volumes to one of three standard input workflows (50 µL to 400 µL, up to 1 mL, up to 2 mL) and follow the work instructions for either manual or semi-automated sample preparation.
Made for KingFisher purification systems The MagMAX DNA Multi-Sample Ultra 2.0 Kit is optimized for use on the moderate-throughput KingFisher Duo Prime (Cat. No. 5400110) and the higher-throughput KingFisher Flex (Cat. No. 5400630) purification systems. Users can process up to 400 µL of sample volume in high-throughput SBS 96-well deep-well microtiter plates using the Flex system. For smaller sample batches, users can process 12 samples using the Duo Prime system. Larger sample input volumes (1–2 mL), which are more challenging to process, are purified in SBS 24-well deep-well microtiter plates for either 24 samples on the Flex or 6 samples on the Duo Prime system. KingFisher instrument BindIt software scripts for all sample types and both Flex and Duo Prime systems can be downloaded below and installed onto the KingFisher instruments.
Working with higher throughput sample numbers? The same MagMAX DNA Multi-Sample Ultra 2.0 Kit reagents are available as automation-friendly stand-alone reagents. MagMAX DNA Multi-Sample Ultra 2.0 stand-alone reagents are individually bottled in larger reagent volumes to make your high-thoughput workflows easier to handle. When purchased together as a sets of 6 stand-alone reagents, users will have sufficient volume of MagMAX DNA Multi-Sample Ultra 2.0 reagents to process 2000 50-200 uL samples or 1000 200-400 ul samples.
• 4.5 mL MagMAX DNA Multi-Sample Ultra 2.0 Proteinase K, store at room temperature • 4.5 mL MagMAX DNA Multi-Sample Ultra 2.0 Binding Beads, store at room temperature • 110 mL MagMAX DNA Multi-Sample Ultra 2.0 Wash I Solution, store at room temperature • 45 mL MagMAX DNA Multi-Sample Ultra 2.0 Lysis/Binding Solution, store at room temperature • 12 mL MagMAX DNA Multi-Sample Ultra 2.0 Elution Solution, store at room temperature • 4.5 mL MagMAX DNA Multi-Sample Ultra 2.0 Enhancer Solution, store (at room temperature