Pierce™ Anti-DYKDDDDK Magnetic Agarose
Pierce™ Anti-DYKDDDDK Magnetic Agarose
Thermo Scientific™

Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins
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Catalog NumberQuantity
A367974 mL
A3679820 mL
Catalog number A36797
Price (USD)
482.65
Online Exclusive
538.00
Save 55.35 (10%)
Each
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Quantity:
4 mL
Price (USD)
482.65
Online Exclusive
538.00
Save 55.35 (10%)
Each
Add to cart

Thermo Scientific Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells. The amino acid sequence DYKDDDDK, commonly known as 'FLAG', is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to a magnetite-embedded agarose core particle.

For protein purification, the magnetic agarose is added to a sample containing DYKDDDDK-tagged proteins with the tag on either the N- or the C-terminus. Captured proteins are then magnetically separated from the supernatant, and non-specifically bound proteins can be washed away before dissociating bound DYKDDDDK-tagged proteins with elution buffer. The magnetic agarose is removed from the solution using a magnetic stand or an instrument such as the KingFisher Flex Magnetic Particle Processor. Automated instruments are especially useful for higher throughput purifications and screening of purification conditions.

Features include:

  • Specific—unique base beads and highly specific antibody minimizes off-target binding (low non-specific binding)
  • High purity—optimized bind-wash-elute protocol enables high purity
  • High yield—special antibody conjugation method enables high yield
  • Rapid—entire purification protocol typically takes less than 40 mins
  • Economical—purification protocol allows multiple reuses
  • Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)

Characteristics of Pierce Anti-DYKDDDDK Magnetic Agarose:

  • Composition: anti-DYKDDDDK antibody covalently attached to a magnetic, highly crosslinked agarose support
  • Magnetization: ferrimagnetic with low remanence
  • Bead size: 10–40 μm
  • Bead concentration: 25% slurry in phosphate buffered saline, 0.01% Tween-20 detergent, 0.02% sodium azide, pH 7.2
  • Binding capacity: ≥3.2 mg DYKDDDDK-tGFP-His protein (∼32 kDa)/mL settled beads
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Bed Volume1 mL
Capacity4 mL
FormLiquid
FormatLiquid
FormulationSlurry: 25%
Particle Size25 to 30 μm
Product LinePierce™
Product TypeMagnetic Agarose
Quantity4 mL
Shipping ConditionWet Ice
Stationary PhaseAnti-DYKDDDDK monoclonal antibodies
MatrixMagnetic Agarose
Unit SizeEach
Contents & Storage
1 mL settled beads. 4 mL supplied as a 25% suspension in PBS containing 0.02% sodium azide.

Store at 2–8°C.