CTS™ OpTmizer™ T-Cell Expansion SFM, no phenol red, bag format

Catalog number:  A3705003

 Related applications:

Primary Cell Culture

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Gibco CTS OpTmizer T-Cell Expansion SFM supports the growth and expansion of human T lymphocytes. It is a complete serum-free, xeno-free medium consisting of CTS OpTmizer T-Cell Expansion Basal Medium and CTS OpTmizer T-Cell Expansion Supplement, which are mixed together prior to use.

CTS OpTmizer T-Cell Expansion SFM is available with and without phenol red. This version of CTS OpTmizer SFM lacks phenol red, and is available in both bottle and bag format.

Features of CTS OpTmizer T-Cell Expansion SFM include:
• Supports high-density T cell culture (>4 x 106 CD3+ T-cells/mL)
• Supports T-cell activation using Dynabeads magnetic beads, soluble antibodies, and stimulatory antibody-presenting cell protocols
• Similar phenotype, function (e.g., cytokine secretion profile), and viability to T-cells cultured with conventional human AB serum-supplemented medium
• Supports a T-cell phenotype similar to human serum-supplemented medium
• Demonstrates enhanced efficacy and persistence of CART-19 cells when grown in medium supplemented with CTS Immune Cell Serum Replacement (ICSR)

Demonstrated functional performance
Cells grown in CTS OpTmizer T-Cell Expansion SFM with or without human serum demonstrate good cytokine production profiles in polyclonally activated T cells. CTS OpTmizer T-Cell Expansion SFM maintains the desired the CD62L+ central memory T-cell phenotype.

GMP manufactured
The primary manufacturing site for CTS OpTmizer T-Cell Expansion SFM is FDA-registered and has an ISO13485-certified quality management system. The methods and controls used for the manufacturing are in conformity with current Good Manufacturing Practices for medical devices, 21 CFR Part 820, of the regulation. Alternate manufacturing sites are available, providing risk mitigation by helping to ensure security of supply.

CTS OpTmizer T-Cell Expansion SFM has been widely adopted in clinical T-cell research applications including the culture of CAR T-cells. It works with multiple culture formats, including culture flasks, culture bags, and rocking bioreactor systems. CTS OpTmizer T-Cell Expansion SFM was designed to support expansion of cultured human T-cells without additional serum supplementation. Increased performance is generally observed when supplemented with CTS Immune Cell Serum Replacement.

What you get
CTS OpTmizer T-Cell Expansion SFM includes two components: 1 liter of CTS OpTmizer T-Cell Expansion Basal Medium and 26 mLs of CTS OpTmizer T-Cell Expansion Auxiliary Concentrated Medium, which are mixed together to create the complete medium. Full traceability documentation such as Certificate of Origin and Certificate of Analysis are available.
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal Law restricts this device to sale by or on the order of a physician.


Cell Type: T Cells
Product Line: OpTmizer™
Product Size: 1000 mL
Shipping Condition: Room Temperature

Contents & storage

• 1 x 1000 mL bag CTS OpTmizer Expansion Basal Medium, store at 2–8°C, protect from light
• 1 x 26 mL CTS OpTmizer Expansion Supplement, store in the dark at 2–8°C.


Manuals & protocols