Periostin and CCN2 Scaffolds Promote the Wound Healing Response in the Skin of Diabetic Mice.
AuthorsElliott CG, Wang J, Walker JT, Michelsons S, Dunmore-Buyze J, Drangova M, Leask A, Hamilton DW
JournalTissue Eng Part A
PubMed ID30572781
'Nonhealing skin wounds remain a significant burden on health care systems, with diabetic patients 20 times as likely to undergo a lower extremity amputation due to impaired healing. Novel treatments that suppress the proinflammatory signature and induce the proliferative and remodeling phases are needed clinically. We demonstrate that the addition ... More
Massive and parallel expression profiling using microarrayed single-cell sequencing.
AuthorsVickovic S, Ståhl PL, Salmén F, Giatrellis S, Westholm JO, Mollbrink A, Navarro JF, Custodio J, Bienko M, Sutton LA, Rosenquist R, Frisén J, Lundeberg J
JournalNat Commun
PubMed ID27739429
'Single-cell transcriptome analysis overcomes problems inherently associated with averaging gene expression measurements in bulk analysis. However, single-cell analysis is currently challenging in terms of cost, throughput and robustness. Here, we present a method enabling massive microarray-based barcoding of expression patterns in single cells, termed MASC-seq. This technology enables both imaging ... More
A two-hybrid antibody micropattern assay reveals specific
AuthorsDirscherl C, Hein Z, Ramnarayan VR, Jacob-Dolan C, Springer S
JournalElife
PubMed ID30180933
We demonstrate a two-hybrid assay based on antibody micropatterns to study protein-protein interactions at the cell surface of major histocompatibility complex class I (MHC I) proteins. Anti-tag and conformation-specific antibodies are used for individual capture of specific forms of MHC I proteins that allow for location- and conformation-specific analysis by ... More
N-Linked Glycosylation Regulates CD22 Organization and Function.
AuthorsWasim L, Buhari FHM, Yoganathan M, Sicard T, Ereño-Orbea J, Julien JP, Treanor B
JournalFront Immunol
PubMed ID31019513
The organization and clustering of cell surface proteins plays a critical role in controlling receptor signaling; however, the biophysical mechanisms regulating these parameters are not well understood. Elucidating these mechanisms is highly significant to our understanding of immune function in health and disease, given the importance of B cell receptor ... More
Misfolded GPI-anchored proteins are escorted through the secretory pathway by ER-derived factors.
AuthorsZavodszky E, Hegde RS
JournalElife
PubMed ID31094677
We have used misfolded prion protein (PrP*) as a model to investigate how mammalian cells recognize and degrade misfolded GPI-anchored proteins. While most misfolded membrane proteins are degraded by proteasomes, misfolded GPI-anchored proteins are primarily degraded in lysosomes. Quantitative flow cytometry analysis showed that at least 85% of PrP* molecules ... More
Temporal Distribution Patterns of Alexa Fluor 647-Conjugated CeNPs in the Mouse Retina After a Single Intravitreal Injection.
AuthorsWong LL, Barkam S, Seal S, McGinnis JF
JournalAdv Exp Med Biol
PubMed ID31884600
Intravitreal (IVT) injection of ophthalmic therapeutics is the most widely used drug delivery route to the posterior segment of the eye. We employed this method to deliver our inorganic, catalytic antioxidant, cerium oxide nanoparticles (CeNPs), to rodent models of retinal degeneration. A single IVT of CeNPs delays disease progression. Even ... More
Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy.
AuthorsBeliu G, Kurz AJ, Kuhlemann AC, Behringer-Pliess L, Meub M, Wolf N, Seibel J, Shi ZD, Schnermann M, Grimm JB, Lavis LD, Doose S, Sauer M
JournalCommun Biol
PubMed ID31341960
Genetic code expansion (GCE) technology allows the specific incorporation of functionalized noncanonical amino acids (ncAAs) into proteins. Here, we investigated the Diels-Alder reaction between trans-cyclooct-2-ene (TCO)-modified ncAAs, and 22 known and novel 1,2,4,5-tetrazine-dye conjugates spanning the entire visible wavelength range. A hallmark of this reaction is its fluorogenicity - the ... More