Invitrogen™

Collibri™ 3´ mRNA Library Prep Kit for Illumina™ Systems

Catalog number:  A38110024

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Description

The Invitrogen Collibri 3’ mRNA Library Prep Kit is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) platforms. It supports library preparation from polyadenylated RNA in ~4.5 hours. The protocol generates one fragment per transcript, resulting in accurate gene expression values. Sequences obtained are close to the 3’ ends of the transcripts, so the kit is suitable for varying-quality RNA samples, including formalin-fixed paraffin-embedded (FFPE) samples. The majority of inserts are greater than 75 bp in size, corresponding to final library fragment sizes ≥200 bp. Multiplexing of libraries can be carried out using up to 96 single-indexed primers.

For convenience, the Collibri 3’ mRNA Library Prep Kit provides color-coded components for visual tracking of library preparation progress. Inert dyes in the reagents provide visual confirmation of additions and mixing, but do not interfere with enzymatic reactions and do not compromise library prep and sequencing results.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Illumina NGS instruments
Library Type: cDNA Library
Product Line: Collibri™
Product Size: 24 reactions
Sequencing Type: Transcriptome Sequencing
Shipping Condition: Approved for shipment on Wet or Dry Ice
Starting Material: RNA
Workflow Step: Library Generation

Contents & storage

• Collibri Library Prep Kit, store at -20°C
• Collibri Library Cleanup Kit, store at 4°C