Thermo Scientific™

Pro-Detect™ Rapid Antibody Isotyping Assay Kit, rat

Catalog number:  A38551

 Related applications:

Protein Electrophoresis & Western Blotting

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Description

The Thermo Scientific Pro-Detect Rapid Antibody Isotyping Assay Kit - Rat is a single-use, dipstick lateral flow kit for quick, easy determination of rat monoclonal antibody class and subclass identity. This antibody isotyping assay uses membrane-based strips coated with dye-bound conjugated antibodies that provide a visual, color readout of the monoclonal antibody isotype within ten minutes. The kit determines rat IgG1, IgG2a, IgG2b , and IgG2c.

Features of the Pro-Detect Rapid Antibody Isotyping Assay Kit:
Long shelf life—stable for at least one year at 4°C
Simple workflow—dilute antibody sample using provided diluent and dip in the lateral flow strip
Fast—within 5–10 minutes a band appears that indicates the antibody isotype
Sensitive—detect antibodies in tissue culture media or ascites fluid samples down to low ng/mL concentrations
Specific—assay does not cross-react with fetal bovine serum (FBS)
Reliable—results are similar to standard ELISA-based isotyping assays

Each Pro-Detect Rapid Antibody Isotyping assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to a test tube or microplate well and dipping in a lateral flow strip. Gold conjugates embedded in the strip membrane form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated regions of the membrane. Results are displayed as a red band above the printed class or sub-class description, indicating the antibody isotype present.

Determining the subclass of a rat antibody is important when selecting the best purification method. IgG2a, IgG2b, and IgG2c can be purified with Protein A columns at a pH of 7–8. IgG1 isotypes bind best to Protein A at a slightly higher pH of 8–9.

The Pro-Detect Rapid Antibody Isotyping Assay Kit is compatible with both tissue culture supernatant and mouse ascites fluid, and is more sensitive than conventional latex bead-based dipstick assays and much faster than ELISA-based isotyping assays. The kit requires only 0.5 µL ascites fluid, 2–22 µL of cell culture supernatant or 1.5 ng of purified antibody.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bulk or Custom Form: Bulk Custom Form 1
Target Specificity: Target-Specific
Assay Type: Isotyping Assay
Detection Method: Chromogenic
Product Line: Pro-Detect™
Product Size: 10 tests
Technique: Lateral Flow Rapid Assay
Shipping Condition: Wet Ice

Contents & storage

• 10 Rapid Antibody Isotyping Assay Strips 1
• 10 Rapid Antibody Isotyping Assay Strips 2
• Sample Diluent, 15 mL

Sufficient for 10 isotype determinations

Store at 2–8°C.

Documents

Manuals & protocols