SuperSignal™ West Atto Ultimate Sensitivity Substrate
SuperSignal™ West Atto Ultimate Sensitivity Substrate
Thermo Scientific™

SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein detection. SuperSignalRead more
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Catalog NumberQuantity
A3855420 mL
A38555100 mL
A38556200 mL
Catalog number A38554
Price (USD)/ Each
127.65
Online exclusive
138.00 
Save 10.35 (8%)
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Quantity:
20 mL
Price (USD)/ Each
127.65
Online exclusive
138.00 
Save 10.35 (8%)
Add to cart
SuperSignal™ West Atto Ultimate Sensitivity Substrate
Catalog numberA38554
Price (USD)/ Each
127.65
Online exclusive
138.00 
Save 10.35 (8%)
-
Add to cart

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein detection. SuperSignal West Atto substrate provides the highest-level sensitivity and better signal-to–noise ratios compared to other commercially available high-performance horseradish peroxidase (HRP) substrates.

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate characteristics include:

  • Sensitivity: low-femtogram to high-attogram
  • Stability: 48 hours for working solution, one year for kit at 4°C
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: up to six hours
  • Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
  • Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

Compare and learn more about our western blot chemiluminescent substrates ›

View all HRP substrates available ›

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is designed for superior sensitivity and signal-to–noise ratios for very low abundant targets or precious samples in western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Atto substrate enables detection of target proteins in amounts that are too small to be seen with typical high performance ECL substrates.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeSubstrate
Detection MethodChemiluminescence
FormLiquid
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LineSuperSignal™
Quantity20 mL
SubstrateHorseradish Peroxidase
SensitivityLow-femtogram to high-attogram
Sufficient For2 Mini-Gel Blots, 200 sq. cm of Membrane
Shipping ConditionWet Ice
Unit SizeEach
Contents & Storage
SuperSignal West Atto Substrate / SuperSignal West Atto Stable Peroxide

• Substrate Solution, 10 mL
• Stable Peroxide Solution, 10 mL

Sufficient for two mini-gel blots; 200 cm2 of membrane.

Store reagents at 2–8°C.