Invitrogen™

Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems, with UD indexes (Set A, 1-24)

Catalog number:  A38602024

Related applications:

DNA Sequencing

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Description

The Invitrogen Collibri PCR-Free ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri PCR-Free ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Supports PCR-free protocols starting with as little as 100 ng of DNA
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PCR-Free ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step (see figure below for visual tracking). The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.The Collibri PCR-Free ES DNA Library Prep Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact DNA. The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PCR-Free ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Comments: The product contains modules A38575024, A38587024, A43190024
For Use With (Equipment): HiSeq X,HiSeq2500,HiSeq3000,HiSeq4000,MiSeq or iSeq,NextSeq 550,NovaSeq 6000
Library Type: Fragment Library
Product Line: Collibri™
Product Size: 24 preps
Sequencing Type: Genome & DNA Sequencing
Shipping Condition: Dry Ice and Cool Packs
Starting Material: DNA
Workflow Step: Library Generation

Contents & storage

• Collibri PCR-free ES DNA Library Prep Kit, store at -20°C
• Collibri DNA Library Cleanup Kit, store at 4°C
• Collibri DNA UD Indexes, store at -20°C