Invitrogen™

Collibri™ ES DNA Library Prep Kit for Illumina Systems, with CD indexes

Catalog number:  A38605024

Related applications:

DNA Sequencing

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Description

The Invitrogen Collibri ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.

The Collibri ES DNA Library Prep Kit is designed for fast and convenient library preparation from a wide range of inputs (1–500 ng) starting from intact DNA. Additionally, a PCR-free version is offered for inputs starting at 100 ng DNA. The entire library preparation protocol takes about three hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): Whole Genome Sequencing, Resequencing, De Novo Assembly
For Use With (Equipment): HiSeq X, HiSeq2500, HiSeq3000, HiSeq4000, MiSeq or iSeq, NextSeq 550, NovaSeq 6000
Format: Kit
Product Type: DNA Library Prep Kit
Quantity: 24 preps
Libraries: Fragment Library
Sequencing Type: Genome & DNA Sequencing
Shipping Condition: Dry Ice
Starting Material: DNA
Workflow Step: Library Generation
Product Line: Collibri™

Contents & storage

• Collibri ES DNA Library Prep Kit, store at -20°C
• Collibri DNA Library Cleanup Kit, store at 4°C
• Collibri DNA CD Indexes, store at -20°C