Collibri™ ES DNA Library Prep Kits for Illumina Systems
Collibri™ ES DNA Library Prep Kits for Illumina Systems
Invitrogen™

Collibri™ ES DNA Library Prep Kits for Illumina Systems

The Invitrogen Collibri ES DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.
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Catalog NumberNo. of ReactionsProduct Type
A3860502424 PrepsES DNA Library Prep Kit for Illumina Systems with CD Indexes
A38605024W24 PrepsES DNA Library Prep Core Kit
A38607096W96 PrepsES DNA Library Prep Core Kit
A3860709696 PrepsES DNA Library Prep Kit for Illumina Systems with CD Indexes
A3860602424 PrepsES DNA Library Prep Kit for Illumina Systems with UD Indexes (Set A, 1-24)
A4360502424 PrepsES DNA Library Prep Kit for Illumina Systems with UD Indexes (Set B, 25-48)
A4360602424 PrepsES DNA Library Prep Kit for Illumina Systems with UD Indexes (Set C, 49-72)
A4360702424 PrepsES DNA Library Prep Kit for Illumina Systems with UD Indexes (Set D, 73-96)
A3860719696 PrepsES DNA Library Prep Kit for Illumina Systems with UD Indexes (Set A-D, 1-96)
Catalog number A38605024
Price (USD)
1,246.00
Each
Add to cart
No. of Reactions:
24 Preps
Product Type:
ES DNA Library Prep Kit for Illumina Systems with CD Indexes
Request bulk or custom format
Price (USD)
1,246.00
Each
Add to cart

The Collibri ES DNA Library Preparation Kit is designed for fast and convenient library preparation from a wide range of inputs (1–500 ng), starting from intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about three hours with a PCR workflow. Additionally, a PCR-free version is available for inputs starting at 100 ng DNA.

The Collibri ES DNA Library Preparation Kit is designed for fast and convenient library preparation from a wide range of inputs (1–500 ng), starting from intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about three hours with a PCR workflow. Additionally, a PCR-free version is available for inputs starting at 100 ng DNA.

The Collibri ES DNA Library Preparation Kit:
• Includes all necessary components in a single kit* for a quick use
• Is empowered on miniaturization liquid handlers to cut on costs
• Provides in-process visual feedback for easier handling
• Is suitable for conventional automation to increase throughput

* The kit consists of enzymatic modification, size selection/clean-up, and indexed adapter-containing modules, except for the Core kit, which enables Collibri combination with desired indexed adapters.

The Collibri ES DNA Library Preparation Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. Moreover, the enzymatic shearing (ES) module of the kit generates a consistently high yield of library from any amount of input DNA (including the PCR-free version), which is enough for library quantification  before sequencing and multiple runs on Illumina instruments.

Applications
The Collibri ES DNA Library Preparation Kit is recommended for:
• Whole genome sequencing
• Resequencing
• De novo assembly

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionThe product contains modules A38579024, A38586024, A43190024
For Use With (Application)Whole Genome Sequencing, Resequencing, De Novo Assembly
For Use With (Equipment)HiSeq X, HiSeq2500, HiSeq3000, HiSeq4000, MiSeq or iSeq, NextSeq 550, NovaSeq 6000
LibrariesFragment Library
No. of Reactions24 Preps
Product LineCollibri™
Product TypeES DNA Library Prep Kit for Illumina Systems with CD Indexes
Quantity24 preps
Sample TypeDNA
Shipping ConditionDry Ice
SpeciesAll
Workflow StepLibrary Generation
FormatKit
Sequencing TypeGenome and DNA Sequencing
Unit SizeEach
Contents & Storage
• Collibri ES DNA Library Prep Kit, store at -20°C
• Collibri DNA Library Cleanup Kit, store at 4°C
• Collibri DNA CD Indexes, store at -20°C

Frequently asked questions (FAQs)

Can indexed adaptors or other modules be purchased separately for the Collibri DNA Library Prep kits?

Indexed adapters and other modules in the kit can be purchased separately. Please refer to thermofisher.com/collibriDNA and contact us for more details.

With the Collibri DNA Library Prep kits, what is the distribution of enzymatically sheared DNA in the library?

Fragment size ranges from 150 bp to 850 bp. For optimal results, we recommend and provide detailed instructions for 350 bp and 550 bp average size library construction.

With the Collibri DNA Library Prep kits, can I use indexed adaptors interchangeably between RNA-seq and DNA-seq applications?

No. They are specifically designed for a product and cannot be used interchangeably.

With the Collibri DNA Library Prep kits, why is my ligation master mix orange instead of red?

Dyes inside the master mix react with CO2 and change the color from red to orange. The mix is still usable when the color changes to orange. To prevent discoloration, avoid keeping it on dry ice.

Are Collibri DNA Library Prep kits compatible with FFPE samples?

Collibri PS DNA Library Prep Kit can be used for library construction from lower-quality DNA (such as FFPE samples). However, the yields of adaptor-ligated molecules are typically lower compared to the yields obtained with high-quality DNA.

Collibri ES DNA Library Prep Kit is not recommended for library construction from FFPE samples.