Gibco™
ExpiSf™ Expression System Starter Kit
Description
The ExpiSf Expression System is a complete chemically-defined baculovirus-insect cell protein expression system that delivers superior yields and consistent performance run after run using a fast, streamlined workflow.The ExpiSf Expression System offers:
• A chemically defined, yeastolate-free, animal origin-free medium for consistent cell growth and protein expression
• Integrated components that work in concert to deliver three times higher protein yields compared to traditional insect expression platforms
• Robust production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
• A fast, simplified workflow that allows you to obtain protein in as little as 6–10 days; half the time of traditional protocols
• Scalable expression that can be applied to cultures from 24-deep well plates to >3 L shake flasks
The components included in the ExpiSf Expression System Starter Kit are: 2 vials of frozen ExpiSf9 Cells, 1 liter of ExpiSf CD Medium, ExpiSf Enhancer sufficient for 1 liter of culture, 1 mL ExpiFectamine Sf Transfection Reagent, 500 mL Opti-MEM Reduced Serum Medium, 0.5 mL MAX Efficiency DH10Bac Competent Cells, 1 Bac-to-Bac Vector Kit containing the pFastBac 1 Expression Vector and pFastBac 1-Gus Positive Control Vector. All components of the ExpiSf Expression System are also available for purchase separately.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Cell Line: | Sf9, SFM Adapted |
|---|---|
| Delivery Type: | Baculovirus |
| Product Line: | ExpiSf™ |
| Promoter: | Polyhedrin |
| Protein Tag: | Untagged |
| Shipping Condition: | Approved for shipment at Room Temperature or on Wet or Dry Ice |
| Cloning Method: | Restriction Enzyme ⁄ MCS |
Contents & storage
• 1000 mL bottle ExpiSf CD Medium, store in the dark at 2–8°C
• 4 x 1 mL ExpiSf Enhancer, 2–8°C
• 1 mL ExpiFectamine Sf Transfection Reagent, 2–8°C
• 2 x 1 vial ExpiSf9 Cells, store in vapor-phase liquid nitrogen
• 500 mL Opti-MEM, 2–8°C
• 10 µg pFastBac1 vector, 2–8°C
• 4 ng pFastBac1-Gus control vector, 2–8°C
• 5 x 0.1 mL MAX Efficiency DH10Bac Competent Cells, –80°C
• 24 x 125-mL Single-Use Nalgene PETG Erlenmeyer Flasks, store at room temperature
• 4 x 1 mL ExpiSf Enhancer, 2–8°C
• 1 mL ExpiFectamine Sf Transfection Reagent, 2–8°C
• 2 x 1 vial ExpiSf9 Cells, store in vapor-phase liquid nitrogen
• 500 mL Opti-MEM, 2–8°C
• 10 µg pFastBac1 vector, 2–8°C
• 4 ng pFastBac1-Gus control vector, 2–8°C
• 5 x 0.1 mL MAX Efficiency DH10Bac Competent Cells, –80°C
• 24 x 125-mL Single-Use Nalgene PETG Erlenmeyer Flasks, store at room temperature
