Sequencing Analysis Software 8, initial license

Sequencing Analysis Software 8 enables you to basecall, trim, display, edit, and print data from our entire line of capillary DNA sequencing instruments for data analysis and quality control.

• Determine the quality of your data using informative metrics from basecalling quality values
• Accelerate quality control using analysis reports with analysis statistics
• Filter out low-quality sequence ends automatically with sequence trimming

Sequencing Analysis Software 8 offers the improved basecaller Smart Deep Basecaller (SDB) (available via subscription). Compared to KB Basecaller (included), Smart Deep Basecaller provides:
• Increased read lengths—more high quality basecalls at 5’ and 3’ ends
• More accurate pure and mixed base calls
• Improved basecalling accuracy through artifacts such as dye blobs, N-1 peaks, and mobility shifts
• Improved basecalling accuracy through heterozygous indel variants
• Reduced manual review time—fewer edits and fewer false positives
• Optional enhanced view trace visualization with a cleaned-up baseline and increased resolution in the 3’ end of plasmid (pure base) sequences

Note: SDB basecalling is enabled for data from SeqStudio Flex, SeqStudio, 3730, and 3500 series instruments.

Smart Deep Basecaller requires an annual subscription license. A 90-day demo license is provided with Sequencing Analysis Software 8. After the expiration of this demo license, a subscription license must be purchased.

Smart Deep Basecaller 1 Year License ›
Smart Deep Basecaller 3 Year License ›

Sequencing Analysis Software 8 can continue to be used with KB Basecaller without the Smart Deep Basecaller license.

Easily review sequencing results with quality values
Sequencing Analysis Software 8 enables you to customize and color code the range of the quality values to represent low-, medium-, and high-quality bases. This way, when the basecaller identifies each base and assigns it a quality value, you need only look at the color coding to easily review, discard, or accept it. In addition, the software trims the ends of low-quality bases, grays them out on the user interface for easy identification, and calculates a sample score, which is the average quality value for all the bases in the untrimmed region.

Reduce data screening time
Eliminate manual review of sequencing data batches. With the software's Quality Control (QC) reports, you get read length and sample score (average QV of bases in the clear range) for each sample file, enabling you to sort data by quality. And to make reviewing data even easier, each QC report is hyperlinked back to its source data.