Gibco™

Expi293F™ Inducible Cells

Catalog number:  A39241

 Related applications:

Mammalian Cell Culture

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Description

The Expi293F Inducible cell line is derived from engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The cell line was established by the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. It is a powerful tool for tetracycline-inducible high-yield expression of recombinant proteins.

Features of Expi293 Inducible cells include:
• Suspension, high-density culture in Expi293 Expression Medium
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• Significantly higher protein yields than other HEK293 Tet-inducible cell line alternatives

For inducible expression of your protein of interest, pcDNA5/TO mammalian expression vector (Cat. No. V103320) or an equivalent inducible vector is required for tetracycline-regulated gene expression.

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: Expi293™
Product Size: 1 vial
Shipping Condition: Dry Ice

Contents & storage

1 vial Expi293F Inducible Cells (1x107 cells/vial), store in vapor-phase liquid nitrogen

Documents

Manuals & protocols