BS3 (bis(sulfosuccinimidyl)suberate), No-Weigh™ Format, 10 x 2 mg - FAQs

For crosslinking the antibody to Protein G Dynabeads, please refer to the crosslinking protocol with BS3 (bis(sulfosuccinimidyl)suberate) or Sulpha-DSS, which is an analogue of DSS. The only difference between these two molecules is that DSS is water insoluble and needs to be solubilized in DMSO before use. You can find the protocol at the following link.

Find additional tips, troubleshooting help, and resources within our Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support Center

BS3 (bis(sulfosuccinimidyl)suberate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

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Amine-to-amine crosslinkers are a good choice for your application since amino groups are usually abundant on the surface of proteins. Our most popular amine-reactive homobifunctional crosslinker that is water-soluble is BS3 (Cat. No. A39266). This has a moderate-length (11.4 A) linker that is good for linking adjacent proteins. If you need the ability to cleave the linker with sulfhydryl reducing agents I would suggest DTSSP (Cat. No. 21578). If you think you might need an extra-long crosslinker, then BS(PEG)5 (Cat. No. 21581) or BS(PEG)9 (Cat. No. 21582) are alternatives.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The crosslinks formed by BS3 are stable to SDS/heat treatment as used for gels and blots.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.