Thermo Scientific Pierce Chloroacetamide is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization. Alkylation with chloroacetamide after cysteine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of disulfide bonds. Reducing agents added after alkylation will react with excess chloroacetamide.
The No-Weigh format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. The format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent.