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Ion Torrent™ Dual Barcode Kit 1-96 - FAQs

Ion Torrent™ Dual Barcode Kit 1-96 - FAQs

View additional product information for Ion Torrent™ Dual Barcode Kit 1-96 - FAQs (A39360)

6 product FAQs found

How many Ion AmpliSeq Mouse TCR Beta SR Assay, RNA libraries can I load on each type of chip?

For the Ion 530 Chip, 8 libraries can be loaded. For the Ion 540 Chip, the number of libraries that can be loaded ranges from 8 to 32, depending on the size of the libraries. For the Ion 550 Chip, the number of libraries that can be loaded ranges from 12 to 48.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What types of chips can I use with the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA?

The Ion AmpliSeq Mouse TCR Beta SR Assay, RNA is compatible with the Ion 530 Chip, Ion 540 Chip, and Ion 550 Chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA?

The Ion AmpliSeq Mouse TCR Beta SR Assay, RNA (Cat. No. A45489) consists of the Ion AmpliSeq Mouse TCR Beta-SR Panel, the Ion AmpliSeq Library Kit Plus (Cat. No. 4488990), and the Ion Torrent NGS Reverse Transcription Kit (Cat. No. A45003). Sufficient reagents are provided for the rapid preparation of 24 sample libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How much of each sample is needed for the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA?

The sample RNA input can range from 25 ng to 1 µg.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What types of samples is the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA compatible with?

The Ion AmpliSeq Mouse TCR Beta SR Assay, RNA is compatible with a vast array of research sample types, including FFPE tissue, fresh-frozen (FF) tissue, sorted T cells, whole blood, periperal blood leukocytes (PBLs), and periperal blood mononuclear cells (PBMCs).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA?

The Ion AmpliSeq Mouse TCR Beta SR Assay, RNA (Cat. No. A45489) is a robust, targeted next-generation sequencing (NGS) assay designed to accurately identify and measure the clonal expansion of T lymphocytes by targeting the complementarity-determining region 3 (CDR3) of the T-cell receptor (TCR) gene locus from total RNA input.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.