Thermo Scientific™

Pierce™ Chromogenic Endotoxin Quant Kit

Catalog number: A39552
Thermo Scientific™

Pierce™ Chromogenic Endotoxin Quant Kit

Catalog number: A39552
Catalog Number
A39552
Unit Size
60 reactions
Price (USD)
376.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
240 reactions
Price (USD)
968.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
30 reactions
Price (USD)
204.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A3955260 reactions
376.00
Your Price:
***
A39553240 reactions
968.00
Your Price:
***
A39552S30 reactions
204.00
Your Price:
***
Product Overview
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The Thermo Scientific Pierce Chromogenic Endotoxin Quant Kit is a highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acid, or antibody sample using the amebocyte lysate assay. The kit enables detection within two linear sensitivity ranges of 0.01–0.1 EU/mL and 0.1–1.0 EU/mL.

Features and benefits of the Pierce Chromogenic Endotoxin Quant Kit include:
Highly sensitive with a broad range—detect as little as 0.01 EU/mL to 1 EU/mL
Specific—no interference from ß-glucans and suitable for wide range of samples, including protein, vaccine, plasmid, DNA, RNA
Fast—perform assay in as little as 20 minutes
End-point chromogenic assay—measure with a standard spectrophotometer or plate reader at 405–410 nm

The Pierce Chromogenic Endotoxin Quant Kit is an end-point chromogenic endotoxin detection assay based on the amebocyte lysate method, which measures endotoxin through the interaction of the endotoxin with the proenzyme Factor C found in circulating amebocytes of the horseshoe crab. The proteolytic activity of this proenzyme is activated in the presence of lipopolysaccharides (endotoxins) derived from the outer cell membrane of gram-negative bacteria such as E.coli. Endotoxin levels are determined by measuring the activity of Factor C in the presence of a synthetic peptide substrate that releases p-nitroaniline (pNA) after proteolysis, producing a yellow color that can be measured at an absorbance of 405 nm.

Endotoxin levels in the samples are accurately determined using the included endotoxin standard of known concentration that is derived from E.coli strain O111: B4. Determining endotoxin levels is important to assess the efficiency of endotoxin removal methods and prevent endotoxic shock, inflammation, and/or sepsis in tissue culture cells and animals injected with endotoxin-contaminated proteins.

Applications: quantitation of endotoxin levels in a protein, peptide, antibody, or nucleic acid samples

Related products:
Pierce High Capacity Endotoxin Removal Resin
Pierce High Capacity Endotoxin Removal Spin Columns

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
60 Reactions
Sufficient For
60 microplate-well assays containing 0.01 to 1 endotoxin units/mL
Form
Kit
Product Line
Pierce™
Type
Endotoxin Quantitation Kit
Assay Range
0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL
Assay Sensitivity
0.01 EU/mL
Assay
End-point Chromogenic
Detection Method
Colorimetric
Shipping Condition
Wet Ice

Contents & Storage

• Lyophilized amebocyte lysate
• E.coli endotoxin standard
• Chromgenic substrate
• Endotoxin-free water

Store at 2–8°C upon receipt.

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