Neurobasal™ PhysioPlus Medium

Neurobasal PhysioPlus Medium is an advanced cell culture medium for maturation and maintenance of primary and hPSC-derived neurons. The medium better replicates the central nervous system (CNS) extracellular environment and supports reliable neuronal activity that arises earlier in cultures and is sustained over long term culture durations. The medium is recommended in combination with B-27 Plus Supplement and helps provide consistent neural activity while maintaining exceptional cell viability, ensuring dependable experimental results in a physiologically relevant system.

Key Features & Benefits

Supports robust performance in functional assays

Neurobasal PhysioPlus Medium is optimized to support reliable neuronal activity and functional maturation across a wide range of neurobiology workflows, including multielectrode array (MEA) and calcium imaging applications.

• Promotes earlier onset of spontaneous neuronal activity compared to traditional neuronal cell culture media
• Enables a higher proportion of electrically active neurons across wells and plates
• Supports sustained and stable network activity during long-term culture, increasing the useful experimental window of your neuronal cultures

Designed to better reflect the physiological CNS environment

Unlike conventional neuronal culture media optimized primarily for survival, Neurobasal PhysioPlus Medium was formulated to more closely mimic aspects of the extracellular environment of the central nervous system.

• Physiologically informed formulation designed to support more in vivo-like neuronal behavior and help improve biological relevance of in vitro neuronal models
• Optimized glucose concentration
• Optimized neuroactive amino acid composition
• Osmolarity aligned more closely with cerebrospinal fluid (CSF)

• Supports electrophysiological properties, such as resting membrane potentials, spontaneous action potentials, and rheobase, more representative of native neuronal physiology, verified using whole-cell patch clamp recordings

Supports neuronal health, maturation, and long-term culture maintenance

Neurobasal PhysioPlus Medium was developed to support both neuronal function and cell health throughout extended culture durations.

• Optimized nutrient composition supports sustained neuronal viability without compromising activity
• Enables maintenance of healthy neuronal cultures for longer maturation and extended experimental timelines, including studies requiring more than a month in culture

• Promotes extensive neurite outgrowth and neuronal network development

Optimized for modern neurobiology workflows

Neurobasal PhysioPlus Medium supports a broad range of neuronal cell culture applications and is recommended for use with B-27 Plus Supplement for optimal performance.

• Compatible with both hPSC-derived neuronal models and primary rodent neurons
• Supports applications including disease modeling, neurotoxicity studies, neuronal maturation, and functional screening
• Available with or without phenol red to support imaging-based workflows
• Enables researchers to generate more consistent and physiologically relevant experimental results

Built on the trusted Neurobasal legacy

Neurobasal PhysioPlus Medium builds upon the established Neurobasal platform trusted by neuroscientists for more than 30 years, delivering enhanced functional performance while maintaining the reliability and usability researchers expect from Gibco neuronal culture systems.