Thermo Scientific™

SureQuant™ AKT Isoform IP and MS Sample Preparation Module

Catalog number:  A40091

 Related applications:

Protein Mass Spectrometry Analysis

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The Thermo Scientific SureQuant AKT Isoform IP and MS Sample Preparation Module provides a complete reagent solution for the multiplexed enrichment and sample preparation of AKT1, AKT2, and AKT3 for mass spectrometry (MS).

SureQuant AKT Isoform IP and MS Sample Preparation Module features include:
Complete—includes all of the reagents necessary for sample lysis, immuno-enrichment, and MS sample preparation
Validated—antibodies validated for specificity and background
Optimized—procedure simplified and condensed to prepare samples after immunoprecipitation in four hours
Multiplex—able to immuno-enrich AKT1, AKT2, and AKT3 isoforms in a single reaction

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and AKT plays a vital role in the signaling cascade. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This module is ideal for immuno-enrichment of the AKT1, AKT2, and AKT3 isoforms for use with the SureQuant quantitation modules.

The SureQuant AKT Isoform IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, where non-specific proteins are removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

This kit can be used with the either the SureQuant AKT Isoform Absolute Quantitation Module or the SureQuant AKT Isoform Relative Quantitation Module for protein targeted quantitation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Mass Spectrometry
Final Product: Peptide
For Use With (Equipment): Mass Spectrometer, Thermo Scientific™ Q Exactive Mass Spectrometer
Product Size: 10 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice
Starting Material: Protein samples
Workflow Step: Target Selection


Manuals & protocols