Informational:Informational:This product has been discontinued as of December 31st, 2021. Please note that all individual kit components are still available for sale; however, CM4000 is no longer recommended for the maintenance of 3D hepatic spheroids. For information regarding our updated protocol for 3D hepatic spheroids please refer to our updated user guide found here. If you have any questions, please contact our technical support team at

Hepatic Spheroid Kit

Catalog number: A41390

Hepatic Spheroid Kit

Catalog number: A41390
Catalog Number
Unit Size
1 kit
Price (USD)
Catalog NumberUnit SizePrice (USD)
A413901 kitContact Us ›
Product Overview
Citations & References
Additional Information
The Gibco Hepatic Spheroid Kit is the first off-the-shelf kit for generation of hepatic spheroids in your own lab.

Maintain cells in culture longer—up to 21 days, >4X longer than cells last in monolayer culture
Design your own schedule—cells are ready for plating when you are
Interrogate pathways of interest—applicable for toxicity, metabolism, or disease modeling

What you get:
• Spheroid-qualified hepatocytes
• Hepatocyte Thaw Medium
• Plating supplement (Cocktail A + Dexamethasone)
• Maintenance supplement (Cocktail B + Dexamethasone)
• Fetal bovine serum
• Williams' E Medium, no phenol red
• Nunclon Sphera 96-well U-bottom plates

Please note that components are shipped separately due to differences in storage temperatures.

Simplified protocol
Cryopreserved cells can be thawed and plated at your convenience, in as little sa 60 minutes. Try it on your own, or request a demo with an experienced scientist.

Stringent quality control
Our high standards help ensure only the best spheroid-forming lots become available, maximizing your chance of success. In addition, each lot is tested for:
• Albumin production in monolayer and spheroid culture
• Phase I enzyme induction
• Bile duct formation (CFDA staining)
• Proper morphology
• Viability >75%
• Attachment efficiency
• Monolayer formation and integrity

Important! For best results, do not attempt hepatic spheroid formation in low attachment plates from other sources. Hepatocyte lots are qualified in Nunclon Sphera plates only and use of other plates can lead to variability in spheroid formation.

Flexible readouts
Analyze single spheroids, or pool several for detection in assays such as gene expression, immunostaining, viability, or CYP activity.


Cell Type
Donor Source
Single Donor
Hepatic Spheroid Kit
Shipping Condition
Cells - dry shipperPlates - room tempMedia / reagents - dry ice

Contents & Storage

• Spheroid-qualified hepatocytes (1 mL, liquid N2 vapor phase only)
• Hepatocyte thaw medium (45 mL, 2–8°C)
• Plating supplement (Cocktail A + Dexamethasone) (18 mL, 2–8°C)
• Maintenance supplement (Cocktail B + Dexamethasone) (20 mL, 2–8°C)
• Fetal bovine serum (2 x 25 mL, -5 to -30°C)
• Williams' E Medium, no phenol red (2 x 500 mL, 2-8°C. Protect from light.)
• 8 Nunclon Sphera 96-well U-bottom plates