High-Intensity Perfusion CHO Medium, 370 L - FAQs

In general, High-Intensity Perfusion (HIP) CHO Medium was not intended to be paired with concentrated feeds and as such may not benefit from them the way that other media intended for fed-batch application do.

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Cells that are grown in or adapted to, and frozen in Gibco media such as CD CHO, CH OptiCHO, FortiCHO, Dynamis, and ExpiCHO seem to readily adapt to High-Intensity Perfusion (HIP) CHO Medium. However, adaptation should always be first demonstrated in flasks as suggested in the product manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019412_High-IntensityPerfusionCHO_Medium_UG.pdf). Only when it is proven that a cell clone suffers no growth lag should it be thawed directly into HIP CHO Medium and/or switched into HIP CHO Medium mid-process workflow.

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When using a CHO cell line with a selection system other than the glutamine synthetase (GS) system, we recommend adding 2-8 mM L-Glutamine or GlutaMAX Supplement to High-Intensity Perfusion (HIP) CHO Medium. Additionally, we recommend making sure that glucose levels do not fall below 2 g/L in culture by supplementing with glucose as needed. Also, if clumping becomes an issue, then anti-clumping agent (ACA) can be used.

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Fed-batch oriented formulations typically perform best when paired with a feed. High-Intensity Perfusion (HIP) CHO Medium, by comparison, is formulated at a higher native concentration and assumes constant medium exchange without the addition of other feeds. Further, the osmolality of HIP CHO Medium at standard concentration is much higher than that of a typical fed-batch medium with the understanding that typical perfusion results in a lower osmolality. Lastly, HIP CHO Medium supports being reconstituted at multiple concentrations to better support the wide array of perfusion operating environments and seed train vs production needs.

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There are many situations where you may want to consider using High-Intensity Perfusion (HIP) CHO Medium:
- Want to switch to perfusion with transfected CHO suspension culture
- Want a single platform with minimal complexity
- Desire high process flexibility
Additionally, HIP CHO medium allows sustained continuous production perfusion for long duration.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.