DLL4 Recombinant Human Protein, hIgG1-Fc Tag

Catalog number:  A42511

Related applications:

Protein Assays and Analysis

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Description

Delta-like 4 (Drosophila) (DLL4) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant human protein is expressed from a DNA sequence encoding the extracellular domain (Met 1-Pro 524) of human DLL4 pre-protein (NP_061947.1) fused to the Fc region of human IgG1 at the C-terminus.

N-terminal Sequence Analysis: Ser 27.

Activity: measured by its binding ability in a functional ELISA. Immobilized human DLL4 at 10 µg/mL (100 µL/well) has been shown to bind biotinylated mouse NOTCH1-His. The EC50 of biotinylated mouse NOTCH1-His is 40 ng/mL.

Formulation: lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
Delta-like protein 4 (DLL4) is a type I membrane-bound Notch ligandcharacterized by an extracellular region containing several EGF-likedomains and a DSL domain required for receptor binding, and issuggested to play a key role in vascular development and tumorangiogenesis. The Notch pathway is an evolutionary conservedintercellular signaling pathway involved in numerous biological processesincluding cell fate determination, cellular differentiation, proliferation, survival, and apoptosis. In mammalian cells, five Notch ligands (Jagged1, 2, DLL1, 3, 4) and four Notch receptors (Notch1-4) have been identified, and ligand-receptor interactions results in proteolysis and translocation of the Notch intracellular domain. As a ligand for Notch1 and Notch 4, DLL4 is selectively expressed in the developing endothelium and in some tumor endothelium, and is induced by vascular endothelial growth factor (VEGF)-A and hypoxia. In recent studies, it has been revealed that this DLL4 inhibition may paradoxically lead to increased angiogenesis but poor tumor growth because of the non-functional neovascularization, and constitutive expression of DLL4 may leads to a lethal lymphoproliferative disease. Accordingly, this may provide a new therapeutic approach for certain carcinomas.

You may also be interested in the following DLL4 products:
Gene Expression Assays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Form: Lyophilized
Protein: delta-like 4 (Drosophila)
Research Category: Angiogenesis, Drug Development, Oncology, Signal Transduction
Species: Human
Molecular Weight: 81 kDa
Purity: ≥ 90% by SDS-PAGE
Protein Subtype: Neurotrophic Factors
Gene Alias: DLL4, MGC126344, hdelta2
Gene Symbol: DLL4
Validated Application: Assay Standard
Protein Family: Other Proteins
Expression System: Mammalian
Protein Tag: IgG1 Fc Tag

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.