IL13 Recombinant Cynomolgus Protein, N-His Tag

Catalog number:  A42582

Related applications:

Protein Assays and Analysis

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Description

Interleukin 13 (IL13), recombinant cynomolgus protein is supplied as a lyophilized powder. It is suitable for use in performing cell-based assays and protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of cynomolgus (Macaca fascicularis) IL-13 (ABG75889.1) (Ser 21-Asn 132) fused to a polyhistidine tag at the N-terminus.

Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized cynomolgus IL-13 at 4 µg/ml (100 µl/well) can bind human IL13RA1 with a linear ranger of 0.156-2.5 µg/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The specific activity is 5-20 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Form: Lyophilized
Protein: interleukin 13
Research Category: Immunology, Inflammation, Signal Transduction
Species: Monkey
Molecular Weight: 14.5 kDa
Purity: ≥ 90% by SDS-PAGE
Protein Subtype: Interleukins
Gene Alias: IL-13
Gene Symbol: IL13
Validated Application: Assay Standard
Protein Family: CD Proteins
Expression System: Mammalian
Protein Tag: N-His

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.