Invitrogen™

TrueTag™ Donor DNA Kit, RFP

Catalog number: A42993
Invitrogen™

TrueTag™ Donor DNA Kit, RFP

Catalog number: A42993
Catalog Number
A42993
Unit Size
10 reactions
Price (USD)
Price: 670.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A4299310 reactions
Price: 670.00
Your Price:
-
Product Overview
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The Invitrogen TrueTag Donor DNA Kit, RFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

Benefits of the TrueTag Donor DNA Kit includes:
• One-step PCR amplification to generate donor templates—no cloning required
• Generate transfection-ready DNA donors in hours instead of days
• Enrich up to 100% RFP-tagged cells

The TrueTag Donor DNA Kit, RFP, provides everything you need to produce a high-quality DNA donor to tag your gene with a selection marker and RFP. Simply provide two locus-specific primers to add homology arms, PCR-amplify, and purify for a transfection-ready donor in hours, not days. Each kit is designed to enable you to:
• Tag the carboxy- (C-) or amino- (N-) terminus of your gene of interest
• Choose from puromycin or blasticidin selection markers—whatever best suits your cell model
• Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment

Included in the kit
• Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
• Phusion Flash High-Fidelity PCR Master Mix
• PCR cleanup columns and buffers
• Positive control primers to tag human beta-actin (ACTB)

Intuitive software to rapidly design gene-specific reagents
Target-specific gene editing reagents are required for proper knock-in of your fluorescent tag. The Invitrogen TrueDesign Genome Editor web-based software was specifically created to:
1. Identify your target gene transcript
2. Designate your insertion site
3. Design and enable easy ordering of all necessary components

Required reagents available via TrueDesign software (not included in kit)
• CRISPR guide RNA or TALENs designed adjacent to N- or C-terminus
• Oligos to PCR-amplify your donor and add appropriate homology arms
• Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
• Verification primers to validate proper insertion of the TrueTag DNA Donor

Also required and available for purchase separately or as part of TrueDesign workflow:
• TrueCut Cas9 Protein v2, TrueCut HiFi Cas9 Protein, or GeneART CRISPR mRNA
• CRISPRMAX Transfection Reagent or MessengerMAX Transfection Reagent

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description
Tag: RFP
Format
Kit
For Use With (Application)
Fluorescent gene tagging, cell line development
Label or Dye
RFP
No. of Reactions
10 reactions
Product Line
TrueTag™
Product Type
Donor DNA Kit
Shipping Condition
Approved for shipment on Wet or Dry Ice

Contents & Storage

• TrueTag Assembly Kit, store at -20°C
• TrueTag Cleanup Kit, store at room temperature

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