TrueTag™ Donor DNA Kit, RFP

Catalog number: A42993
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TrueTag™ Donor DNA Kit, RFP

Catalog number: A42993
Have Questions?
Catalog Number
Unit Size
10 reactions
Price (USD)
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A4299310 reactions
Product Overview
Citations & References
Additional Information
The Invitrogen TrueTag Donor DNA Kit, RFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

Benefits of the TrueTag Donor DNA Kit includes:
• One-step PCR amplification to generate donor templates—no cloning required
• Generate transfection-ready DNA donors in hours instead of days
• Enrich up to 100% RFP-tagged cells

The TrueTag Donor DNA Kit, RFP, provides everything you need to produce a high-quality DNA donor to tag your gene with a selection marker and RFP. Simply provide two locus-specific primers to add homology arms, PCR-amplify, and purify for a transfection-ready donor in hours, not days. Each kit is designed to enable you to:
• Tag the carboxy- (C-) or amino- (N-) terminus of your gene of interest
• Choose from puromycin or blasticidin selection markers—whatever best suits your cell model
• Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment

Included in the kit
• Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
• Phusion Flash High-Fidelity PCR Master Mix
• PCR cleanup columns and buffers
• Positive control primers to tag human beta-actin (ACTB)

Intuitive software to rapidly design gene-specific reagents
Target-specific gene editing reagents are required for proper knock-in of your fluorescent tag. The Invitrogen TrueDesign Genome Editor web-based software was specifically created to:
1. Identify your target gene transcript
2. Designate your insertion site
3. Design and enable easy ordering of all necessary components

Required reagents available via TrueDesign software (not included in kit)
• CRISPR guide RNA or TALENs designed adjacent to N- or C-terminus
• Oligos to PCR-amplify your donor and add appropriate homology arms
• Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
• Verification primers to validate proper insertion of the TrueTag DNA Donor

Also required and available for purchase separately or as part of TrueDesign workflow:
• TrueCut Cas9 Protein v2, TrueCut HiFi Cas9 Protein, or GeneART CRISPR mRNA
• CRISPRMAX Transfection Reagent or MessengerMAX Transfection Reagent

For Research Use Only. Not for use in diagnostic procedures.


Tag: RFP
For Use With (Application)
Fluorescent gene tagging, cell line development
Label or Dye
No. of Reactions
10 reactions
Product Line
Product Type
Donor DNA Kit
Shipping Condition
Approved for shipment on Wet or Dry Ice

Contents & Storage

• TrueTag Assembly Kit, store at -20°C
• TrueTag Cleanup Kit, store at room temperature


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    Frequently asked questions (FAQs)

    Citations & References

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