Targeting Mosquitoes through Generation of an Insecticidal RNAi Yeast Strain Using Cas-CLOVER and Super PiggyBac Engineering in Saccharomyces cerevisiae.
AuthorsBrizzee C,Mysore K,Njoroge TM,McConnell S,Hamid-Adiamoh M,Stewart ATM,Kinder JT,Crawford J,Duman-Scheel M
JournalJournal of fungi (Basel, Switzerland)
PubMed ID37998862
The global deployment of RNAi yeast insecticides involves transitioning from the use of laboratory yeast strains to more robust strains that are suitable for scaled fermentation. In this investigation, the RNA-guided Cas-CLOVER system was used in combination with Piggybac transposase to produce robust Saccharomyces cerevisiae strains with multiple integrated copies ... More
The field of environmental DNA (eDNA) is advancing rapidly, yet human eDNA applications remain underutilized and underconsidered. Broader adoption of eDNA analysis will produce many well-recognized benefits for pathogen surveillance, biodiversity monitoring, endangered and invasive species detection, and population genetics. Here we show that deep-sequencing-based eDNA approaches capture genomic information ... More
SARS-CoV-2 infection triggers pro-atherogenic inflammatory responses in human coronary vessels.
Patients with coronavirus disease 2019 (COVID-19) present increased risk for ischemic cardiovascular complications up to 1 year after infection. Although the systemic inflammatory response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection likely contributes to this increased cardiovascular risk, whether SARS-CoV-2 directly infects the coronary vasculature and attendant atherosclerotic ... More
Estrogen receptor blockade and radiation therapy cooperate to enhance the response of immunologically cold ER+ breast cancer to immunotherapy.
BACKGROUND: Most patients with estrogen receptor positive (ER+) breast cancer do not respond to immune checkpoint inhibition (ICI); the tumor microenvironment (TME) of these cancers is generally immunosuppressive and contains few tumor-infiltrating lymphocytes. Radiation therapy (RT) can increase tumor inflammation and infiltration by lymphocytes but does not improve responses to ... More
Pelvic irradiation induces behavioural and neuronal damage through gut dysbiosis in a rat model.
Effects of the G-quadruplex-binding drugs quarfloxin and CX-5461 on the malaria parasite Plasmodium falciparum.
AuthorsCraven HM,Nettesheim G,Cicuta P,Blagborough AM,Merrick CJ
JournalInternational journal for parasitology. Drugs and drug resistance
PubMed ID38041930
Plasmodium falciparum is the deadliest causative agent of human malaria. This parasite has historically developed resistance to most drugs, including the current frontline treatments, so new therapeutic targets are needed. Our previous work on guanine quadruplexes (G4s) in the parasite's DNA and RNA has highlighted their influence on parasite biology, ... More
Ameliorative effects of Fingolimod (FTY720) on microglial activation and psychosis-related behavior in short term cuprizone exposed mice.
AuthorsLi S,Sakurai K,Ohgidani M,Kato TA,Hikida T
JournalMolecular brain
PubMed ID37438826
Schizophrenia is a psychiatric disorder that affects around 1% of the population in widespread populations, with severe cases leading to long-term hospitalization and necessitation of lifelong treatment. Recent studies on schizophrenia have highlighted the involvement of inflammatory and immunoregulatory mechanisms with the onset of symptoms, and the usage of anti-inflammatory ... More
AuthorsSaleh KK,Switzler C,Hicks MR,Gane L,Gibbs DE,Pyle AD
JournalFrontiers in physiology
PubMed ID37228827
Duchenne muscular dystrophy (DMD) is caused by an out-of-frame mutation in the DMD gene that results in the absence of a functional dystrophin protein, leading to a devastating progressive lethal muscle-wasting disease. Muscle stem cell-based therapy is a promising avenue for improving muscle regeneration. However, despite the efforts to deliver ... More
Wikstroemiaganpi Extract Improved Atopic Dermatitis-Like Skin Lesions via Suppression of Interleukin-4 in 2,4-Dinitrochlorobenzene-Induced SKH-1 Hairless Mice.
Plants of the genus Wikstroemia are used in Chinese traditional medicine to treat inflammatory diseases, such as arthritis, bronchitis, and pneumonia. The present study was designed to determine whether Wikstroemia ganpi (Siebold and Zucc.) Maxim. offers a potential means of treating 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice. Symptoms such ... More
Cycle-dependent sex differences in expression of membrane proteins involved in cerebrospinal fluid secretion at rat choroid plexus.
AuthorsIsraelsen IME,Kamp-Jensen C,Westgate CSJ,Styrishave B,Jensen RH,Eftekhari S
JournalBMC neuroscience
PubMed ID37946101
BACKGROUND: Female sex is a known risk factor of brain disorders with raised intracranial pressure (ICP) and sex hormones have been suggested to alter cerebrospinal fluid (CSF) dynamics, thus impairing ICP regulation in CSF disorders such as idiopathic intracranial hypertension (IIH). The choroid plexus (CP) is the tissue producing CSF ... More
Comparative and integrative single cell analysis reveals new insights into the transcriptional immaturity of stem cell-derived β cells.
AuthorsSchmidt MD,Ishahak M,Augsornworawat P,Millman JR
JournalBMC genomics
PubMed ID38267908
Diabetes cell replacement therapy has the potential to be transformed by human pluripotent stem cell-derived β cells (SC-β cells). However, the precise identity of SC-β cells in relationship to primary fetal and adult β-cells remains unclear. Here, we used single-cell sequencing datasets to characterize the transcriptional identity of islets from ... More
Silibinin Inhibits Cell Ferroptosis and Ferroptosis-Related Tissue Injuries.
Ferroptosis is involved in various tissue injuries including neurodegeneration, ischemia-reperfusion injury, and acute liver injury. Ferroptosis inhibitors exhibit promising clinical potential in the treatment of various diseases. As a traditional chemical, silymarin has been widely used in healthcare and clinical applications to treat liver injuries in which ferroptosis is involved. ... More
Case Report of COVID-19 after Full Vaccination: Viral Loads and Anti-SARS-CoV-2 Antibodies.
AuthorsKomiazyk M,Walory J,Gawor J,Ksiazek I,Gromadka R,Baraniak A
JournalDiagnostics (Basel, Switzerland)
PubMed ID34679513
The introduction of effective vaccines against SARS-CoV-2 is expected to prevent COVID-19. However, sporadic cases of infection in vaccinated persons have been reported. We describe a case of a double-dose vaccinated woman with COVID-19. All stages of infection were observed, from no identification of virus, then the start of the ... More
The evaluation of a rapid microfluidic immunofluorescence antigen test in detecting the infectiousness of COVID-19 patients.
BACKGROUND: A test-based strategy against coronavirus disease 2019 (COVID-19) is one of the measures to assess the need for isolation and prevention of infection. However, testing with high sensitivity methods, such as quantitative RT-PCR, leads to unnecessary isolation, whereas the lateral flow antigen test shows low sensitivity and false negative results. ... More
Transcriptome profiling of barley in response to mineral and organic fertilizers.
AuthorsEsmaeilzadeh-Salestani K,Tohidfar M,Ghanbari Moheb Seraj R,Khaleghdoust B,Keres I,Marawne H,Loit E
JournalBMC plant biology
PubMed ID37193945
BACKGROUND: Nitrogen is very important for crop yield and quality. Crop producers face the challenge of reducing the use of mineral nitrogen while maintaining food security and other ecosystem services. The first step towards understanding the metabolic responses that could be used to improve nitrogen use efficiency is to identify ... More
The immunological impact of adenovirus early genes on vaccine-induced responses in mice and nonhuman primates.
Adenovirus (Ad) is being explored for use in the prevention and treatment of a variety of infectious diseases and cancers. Ad with a deletion in early region 3 (ÎE3) provokes a stronger immune response than Ad with deletions in early regions 1 and E3 (ÎE1/ÎE3). The ÎE1/ÎE3 Ads are more ... More
CREB Ameliorates Osteoarthritis Progression Through Regulating Chondrocytes Autophagy via the miR-373/METTL3/TFEB Axis.
JournalFrontiers in cell and developmental biology
PubMed ID35756079
Osteoarthritis (OA) is a degenerative joint disease characterized by articular cartilage degradation. Dysregulated autophagy is a major cause of OA. However, the underlying mechanism is unclear. Here, we found that the expression of element-binding protein (CREB) was downregulated in both cartilage tissues of OA patients and mouse OA model. In ... More
Postoperative Changes in Systemic Immune Tolerance Following Major Oncologic versus Minor Maxillofacial Surgery.
AuthorsTrumet L,Ries J,Sobl P,Ivenz N,Wehrhan F,Lutz R,Kesting M,Weber M
JournalCancers
PubMed ID37568571
SIMPLE SUMMARY: Surgical procedures can cause a systemic response of the immune system. This is relevant especially for multimodal tumor therapy in which immunotherapy plays an increasing role. How surgical interventions influence the systemic immune tolerance is not yet well understood. Aim of this study was to monitor changes in ... More
Saponins Derived from the Korean Endemic Plant Heloniopsis koreana Inhibit Diffuse-type Gastric Cancer Cells.
[Image: see text] Diffuse-type gastric cancer (GC) is a type of stomach cancer that occurs in small clusters of cells that are widely spread. It does not typically manifest with symptoms until the advanced stages and often goes undetected in routine imaging tests. In addition, there is no specific targeted ... More
The role of pneumococcal extracellular vesicles on the pathophysiology of the kidney disease hemolytic uremic syndrome.
Streptococcus pneumoniae-induced hemolytic uremic syndrome (Sp-HUS) is a kidney disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, and acute kidney injury. This disease is frequently underdiagnosed and its pathophysiology is poorly understood. In this work, we compared clinical strains, isolated from infant Sp-HUS patients, with a reference pathogenic strain D39, for ... More
Progesterone distribution in the trigeminal system and its role to modulate sensory neurotransmission: influence of sex.
AuthorsMaddahi A,Warfvinge K,Holm A,Edvinsson JCA,Reducha PV,Kazantzi S,Haanes KA,Edvinsson L
JournalThe journal of headache and pain
PubMed ID37957603
BACKGROUND: Women are disproportionately affected by migraine, representing up to 75% of all migraine cases. This discrepancy has been proposed to be influenced by differences in hormone levels between the sexes. One such hormone is progesterone. Calcitonin gene-related peptide (CGRP) system is an important factor in migraine pathophysiology and could ... More
Stable Reference Genes for qPCR Analysis in BM-MSCs Undergoing Osteogenic Differentiation within 3D Hyaluronan-Based Hydrogels.
AuthorsHasler J,Hatt LP,Stoddart MJ,Armiento AR
JournalInternational journal of molecular sciences
PubMed ID33276559
Reverse transcription quantitative polymerase chain reaction (RT-qPCR) enables the monitoring of changes in cell phenotype via the high-throughput screening of numerous genes. RT-qPCR is a fundamental approach in numerous research fields, including biomaterials, yet little attention has been given to the potential impact of 3D versus monolayer (2D) cell culture ... More
The impact of viremia on organ failure, biomarkers and mortality in a Swedish cohort of critically ill COVID-19 patients.
AuthorsJärhult JD,Hultström M,Bergqvist A,Frithiof R,Lipcsey M
JournalScientific reports
PubMed ID33785784
The spread of virus via the blood stream has been suggested to contribute to extra-pulmonary organ failure in Coronavirus disease 2019 (COVID-19). We assessed SARS-CoV-2 RNAemia (RNAemia) and the association between RNAemia and inflammation, organ failure and mortality in critically ill COVID-19 patients. We included all patients with PCR verified ... More
Occurrence of Human Enteric Viruses in Shellfish along the Production and Distribution Chain in Sicily, Italy.
AuthorsMacaluso G,Guercio A,Gucciardi F,Di Bella S,La Rosa G,Suffredini E,Randazzo W,Purpari G
JournalFoods (Basel, Switzerland)
PubMed ID34203938
Contamination of bivalve mollusks with human pathogenic viruses represents a recognized food safety risk. Thus, monitoring programs for shellfish quality along the entire food chain could help to finally preserve the health of consumers. The aim of the present study was to provide up-to-date data on the prevalence of enteric ... More
Coating-Free Culture Medium for Establishing and Maintaining Human Induced Pluripotent Stem Cells.
Cell expansion of human pluripotent stem cells (hPSCs) commonly depends on Matrigel as a coating matrix on two-dimensional (2D) culture plates and 3D microcarriers. However, the xenogenic Matrigel requires sophisticated quality-assurance processes to meet clinical requirements. In this study, we develop an innovative coating-free medium for expanding hPSCs. The xenofree ... More
VEGFR3 is required for button junction formation in lymphatic vessels.
Lymphatic capillaries develop discontinuous cell-cell junctions that permit the absorption of large macromolecules, chylomicrons, and fluid from the interstitium. While excessive vascular endothelial growth factor 2 (VEGFR2) signaling can remodel and seal these junctions, whether and how VEGFR3 can alter lymphatic junctions remains incompletely understood. Here, we use lymphatic-specific Flt4 ... More
Quantification of Pork, Chicken, Beef, and Sheep Contents in Meat Products Using Duplex Real-Time PCR.
AuthorsWang Y,Teo E,Lin KJ,Wu Y,Chan JSH,Tan LK
JournalFoods (Basel, Switzerland)
PubMed ID37569240
Accurate methods for meat speciation and quantification are essential for ensuring the supply of safe and wholesome meat and composite products with animal origins to negate the potential associated hazards, aid classification of consignments at the import control system, and thwart food fraud committed for financial gain. To better enhance ... More
The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation.
AuthorsGu X,Nardone C,Kamitaki N,Mao A,Elledge SJ,Greenberg ME
JournalScience (New York, N.Y.)
PubMed ID37616343
Cells use ubiquitin to mark proteins for proteasomal degradation. While the proteasome also eliminates proteins that are not ubiquitinated, how this occurs mechanistically is unclear. Here we found that midnolin promoted the destruction of many nuclear proteins including transcription factors encoded by the immediate-early-genes. Diverse stimuli induced midnolin and its ... More
A viral pan-end RNA element and host complex define a SARS-CoV-2 regulon.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, generates multiple protein-coding, subgenomic RNAs (sgRNAs) from a longer genomic RNA, all bearing identical termini with poorly understood roles in regulating viral gene expression. Insulin and interferon-gamma, two host-derived, stress-related agents, and virus spike protein, induce binding of ... More
Forsythia velutina Nakai extract: A promising therapeutic option for atopic dermatitis through multiple cell type modulation.
BACKGROUND: The urgent need for massively scaled clinical testing for SARS-CoV-2, along with global shortages of critical reagents and supplies, has necessitated development of streamlined laboratory testing protocols. Conventional nucleic acid testing for SARS-CoV-2 involves collection of a clinical specimen with a nasopharyngeal swab in transport medium, nucleic acid extraction, ... More
mRNA-1273 or mRNA-Omicron boost in vaccinated macaques elicits similar B cell expansion, neutralizing responses, and protection from Omicron.
SARS-CoV-2 Omicron is highly transmissible and has substantial resistance to neutralization following immunization with ancestral spike-matched vaccines. It is unclear whether boosting with Omicron-matched vaccines would enhance protection. Here, nonhuman primates that received mRNA-1273 at weeks 0 and 4 were boosted at week 41 with mRNA-1273 or mRNA-Omicron. Neutralizing titers ... More
Development of circulating isolates of Plasmodium falciparum is accelerated in Anopheles vectors with reduced reproductive output.
Anopheles gambiae and its sibling species Anopheles coluzzii are the most efficient vectors of the malaria parasite Plasmodium falciparum. When females of these species feed on an infected human host, oogenesis and parasite development proceed concurrently, but interactions between these processes are not fully understood. Using multiple natural P. falciparum ... More
Differential release of extracellular vesicle tRNA from oxidative stressed renal cells and ischemic kidneys.
AuthorsLee HK,Lee BR,Lee TJ,Lee CM,Li C,O'Connor PM,Dong Z,Kwon SH
JournalScientific reports
PubMed ID35102218
While urine-based liquid biopsy has expanded to the analyses of extracellular nucleic acids, the potential of transfer RNA (tRNA) encapsulated within extracellular vesicles has not been explored as a new class of urine biomarkers for kidney injury. Using rat kidney and mouse tubular cell injury models, we tested if extracellular ... More
Mitochondrial haplotypes affect metabolic phenotypes in the Drosophila Genetic Reference Panel.
AuthorsBevers RPJ,Litovchenko M,Kapopoulou A,Braman VS,Robinson MR,Auwerx J,Hollis B,Deplancke B
JournalNature metabolism
PubMed ID32694676
Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems.
Understanding metabolic heterogeneity is the key to uncovering the underlying mechanisms of metabolic-related diseases. Current metabolic imaging studies suffer from limitations including low resolution and specificity, and the model systems utilized often lack human relevance. Here, we present a single-cell metabolic imaging platform to enable direct imaging of lipid metabolism ... More
Development of an Ultraviolet-C Irradiation Room in a Public Portuguese Hospital for Safe Re-Utilization of Personal Protective Respirators.
JournalInternational journal of environmental research and public health
PubMed ID35457722
Almost two years have passed since COVID-19 was officially declared a pandemic by the World Health Organization. However, it still holds a tight grasp on the entire human population. Several variants of concern, one after another, have spread throughout the world. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) omicron ... More
Differential compartmentalization of BMP4/NOGGIN requires NOGGIN trans-epithelial transport.
AuthorsPhan-Everson T,Etoc F,Li S,Khodursky S,Yoney A,Brivanlou AH,Siggia ED
JournalDevelopmental cell
PubMed ID34051144
Using self-organizing human models of gastrulation, we previously showed that (i) BMP4 initiates the cascade of events leading to gastrulation; (ii) BMP4 signal-reception is restricted to the basolateral domain; and (iii) in a human-specific manner, BMP4 directly induces the expression of NOGGIN. Here, we report the surprising discovery that in ... More
Comparing Transcriptome Profiles of Saccharomyces Cerevisiae Cells Exposed to Cadmium Selenide/Zinc Sulfide and Indium Phosphide/Zinc Sulfide.
AuthorsHorstmann C,Kim K
JournalGenes
PubMed ID33802854
The primary focus of our research was to obtain global gene expression data in bakerâs yeast exposed to sub-lethal doses of quantum dots (QDs), such as green-emitting CdSe/ZnS and InP/ZnS, to reveal novel insights on their unique mechanisms of toxicity. Despite their promising applications, their toxicity and long-lasting effects on ... More
KATP channels are necessary for glucose-dependent increases in amyloid-β and Alzheimer's disease-related pathology.
Elevated blood glucose levels, or hyperglycemia, can increase brain excitability and amyloid-β (Aβ) release, offering a mechanistic link between type 2 diabetes and Alzheimerâs disease (AD). Since the cellular mechanisms governing this relationship are poorly understood, we explored whether ATP-sensitive potassium (K(ATP)) channels, which couple changes in energy availability with ... More
Loss of microRNA-30a and sex-specific effects on the neonatal hyperoxic lung injury.
BACKGROUND: Bronchopulmonary dysplasia (BPD) is characterized by an arrest in lung development and is a leading cause of morbidity in premature neonates. It has been well documented that BPD disproportionally affects males compared to females, but the molecular mechanisms behind this sex-dependent bias remain unclear. Female mice show greater preservation ... More
Heterogeneous vasomotor responses in segments from Göttingen Minipigs coronary, cerebral, and mesenteric artery: A comparative study.
AuthorsSams A,Haanes KA,Holm A,Kazantzi S,Mikkelsen LF,Edvinsson L,Brain S,Sheykhzade M
JournalVascular pharmacology
PubMed ID37730143
SARS-CoV-2 surveillance with environmental surface sampling in public areas.
AuthorsMihajlovski K,Buttner MP,Cruz P,Labus B,St Pierre Schneider B,Detrick E
JournalPloS one
PubMed ID36417446
Contaminated surfaces are one of the ways that coronavirus disease 2019 (COVID-19) may be transmitted. SARS-CoV-2 can be detected on environmental surfaces; however, few environmental sampling studies have been conducted in nonclinical settings. The objective of this study was to detect SARS-CoV-2 RNA on environmental surfaces in public areas in ... More
SARS-CoV-2 in Spent Dialysate from Chronic Peritoneal Dialysis Patients with COVID-19.
BACKGROUND: To date, it is unclear whether SARS-CoV-2 is present in spent dialysate from patients with COVID-19 on peritoneal dialysis (PD). Our aim was to assess the presence or absence of SARS-CoV-2 in spent dialysate from patients on chronic PD who had a confirmed diagnosis of COVID-19. METHODS: Spent PD ... More
Imeglimin profoundly affects the circadian clock in mouse embryonic fibroblasts.
SIMPLE SUMMARY: Early-stage invasive lobular breast cancer (ILC) is characterized by multifocal tumor growth in the breast and a distinct pattern of late metastatic spread to other tissues. ILC cells are hypothesized to initiate metastases by exiting the breast via blood and lymphatic channels early in the disease process and ... More
SUMOylation of the ubiquitin ligase component KEAP1 at K39 upregulates NRF2 and its target function in lung cancer cell proliferation.
Nuclear Factor Erythroid 2-Related Factor 2 (NRF2) is important for the expression of genes associated with oxidative stress. The levels of NRF2 are controlled by Kelch-like ECH-associated protein 1 (KEAP1)-dependent degradation. Although oxidative stress is known to suppress KEAP1 activity to stabilize the levels of NRF2, the mechanism for this ... More
Identification of side effects of COVID-19 drug candidates on embryogenesis using an integrated zebrafish screening platform.
AuthorsErnst A,Piragyte I,Mp AM,Le ND,Grandgirard D,Leib SL,Oates A,Mercader N
JournalScientific reports
PubMed ID37813860
Drug repurposing is an important strategy in COVID-19 treatment, but many clinically approved compounds have not been extensively studied in the context of embryogenesis, thus limiting their administration during pregnancy. Here we used the zebrafish embryo model organism to test the effects of 162 marketed drugs on cardiovascular development. Among ... More
Ectopic expression of murine CD163 enables cell-culture isolation of lactate dehydrogenase-elevating virus 63 years after its discovery.
Arteriviruses are RNA viruses related to coronaviruses but have not yet been associated with human infection. A murine arterivirus, lactate dehydrogenase-elevating virus (LDV), was first described in 1960 and quickly became a promising model for understanding immune failure due to its unique ability to persist in immunocompetent adult mice. However, ... More
Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer.
BACKGROUND: Trastuzumab is a firstâline targeted therapy for human epidermal growth factor receptorâ2 (HER2)âpositive gastric cancer. However, the inevitable occurrence of acquired trastuzumab resistance limits the drug benefit, and there is currently no effective reversal measure. Existing researches on the mechanism of trastuzumab resistance mainly focused on tumor cells themselves, ... More
HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51.
DNA double-stranded breaks (DSBs) are deleterious lesions, and their incorrect repair can drive cancer development(1). HELQ is a superfamily 2 helicase with 3â² to 5â² polarity, and its disruption in mice confers germ cells loss, infertility and increased predisposition to ovarian and pituitary tumours(2â4). At the cellular level, defects in ... More
Renal claudin-14 expression is not required for regulating Mg(2+) balance in mice.
AuthorsFerreira PG,van Megen WH,Tan R,Lee CHL,Svenningsen P,Alexander RT,Dimke H
JournalAmerican journal of physiology. Renal physiology
PubMed ID33818126
Detection of m(6)A RNA modifications at single-nucleotide resolution using m(6)A-selective allyl chemical labeling and sequencing.
AuthorsPeng Y,Meng H,Ge R,Liu S,Chen M,He C,Hu L
JournalSTAR protocols
PubMed ID36112507
As the most abundant internal mRNA modification, N(6)-methyladenosine (m(6)A) was involved in almost all the aspects of RNA metabolism. Here, we introduce our protocol for m(6)A-SAC-seq, which enables the whole transcriptome-wide mapping of m(6)A RNA modification at single-nucleotide resolution with stoichiometry information. m(6)A-SAC-seq relies on selective allyl labeling of m(6)A ... More
A multi-well bioreactor for cartilage tissue engineering experiments.
AuthorsLadner YD,Kasper H,Armiento AR,Stoddart MJ
JournaliScience
PubMed ID37408683
Cartilage tissue engineering necessitates the right mechanical cues to regenerate impaired tissue. For this reason, bioreactors can be employed to induce joint-relevant mechanical loading, such as compression and shear. However, current articulating joint bioreactor designs are lacking in terms of sample size and usability. In this paper, we describe a ... More
A novel method for automated crystal visualization and quantification in murine folic acid-induced acute kidney injury.
AuthorsHamid AK,Pastor Arroyo EM,Lee SS,Wagner CA,Egli-Spichtig D
JournalAmerican journal of physiology. Renal physiology
PubMed ID37881875
Folic acid (FA)-induced acute kidney injury (FA-AKI) is an increasingly prevalent rodent disease model involving the injection of a high dose of FA that culminates in renal FA crystal deposition and injury. However, the literature characterizing the FA-AKI model is sparse and dated in part due to the absence of ... More
Glucocorticoids modify intracranial pressure in freely moving rats.
AuthorsWestgate CSJ,Israelsen IME,Kamp-Jensen C,Jensen RH,Eftekhari S
JournalFluids and barriers of the CNS
PubMed ID37231507
BACKGROUND: Glucocorticoids (GCs) are widely prescribed for a variety of inflammatory diseases, but they are also used to treat raised intracranial pressure (ICP) caused by trauma or oedema. However, it is unclear if GCs independently modulate ICP and if GCs are involved in normal ICP regulation. In this study, we ... More
Candidate SNPs for meat quality and carcass composition in free-range Iberian pigs.
AuthorsPalma-Granados P,Muñoz M,Delgado-Gutierrez MA,Óvilo C,Nuñez Y,Fernández-Barroso MA,Sánchez-Esquiliche F,Ramírez L,García-Casco JM
JournalMeat science
PubMed ID37906998
Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex.
AuthorsPuno MR,Lima CD
JournalCell
PubMed ID35688134
RNA quality control relies on co-factors and adaptors to identify and prepare substrates for degradation by ribonucleases such as the 3â² to 5â² ribonucleolytic RNA exosome. Here, we determined cryogenic electron microscopy structures of human Nuclear Exosome Targeting (NEXT) complexes bound to RNA that reveal mechanistic insights to substrate recognition ... More
Combination of Bempegaldesleukin and Anti-CTLA-4 Prevents Metastatic Dissemination After Primary Resection or Radiotherapy in a Preclinical Model of Non-Small Cell Lung Cancer.
Surgical resection or hypo-fractionated radiation therapy (RT) in early-stage non-small cell lung cancer (NSCLC) achieves local tumor control, but metastatic relapse remains a challenge. We hypothesized that immunotherapy with anti-CTLA-4 and bempegaldesleukin (BEMPEG; NKTR-214), a CD122-preferential IL2 pathway agonist, after primary tumor RT or resection would reduce metastases in a ... More
Environmental DNA monitoring of oncogenic viral shedding and genomic profiling of sea turtle fibropapillomatosis reveals unusual viral dynamics.
Pathogen-induced cancers account for 15% of human tumors and are a growing concern for endangered wildlife. Fibropapillomatosis is an expanding virally and environmentally co-induced sea turtle tumor epizootic. Chelonid herpesvirus 5 (ChHV5) is implicated as a causative virus, but its transmission method and specific role in oncogenesis and progression is ... More
Overcoming Resistance to Immune Checkpoint Inhibitor Therapy Using Calreticulin-Inducing Nanoparticle.
AuthorsChandrasekar SV,Singh A,Ranjan A
JournalPharmaceutics
PubMed ID37376141
Nanoparticles (NPs) have the ability to transform poorly immunogenic tumors into activated âhotâ targets. In this study, we investigated the potential of a liposome-based nanoparticle (CRT-NP) expressing calreticulin as an in-situ vaccine to restore sensitivity to anti-CTLA4 immune checkpoint inhibitor (ICI) in CT26 colon tumors. We found that a CRT-NP ... More
Kdr genotyping (V1016I, F1534C) of the Nav channel of Aedes aegypti (L.) mosquito populations in Harris County (Houston), Texas, USA, after Permanone 31-66 field tests and its influence on probability of survival.
Aedes aegypti (L.) is an important mosquito vector of emerging arboviruses such as Zika, dengue, yellow fever, and chikungunya. To quell potential disease outbreaks, its populations are controlled by applying pyrethroid insecticides, which selection pressure may lead to the development of insecticide resistance. Target site insensitivity to pyrethroids caused by ... More
Comparison of saliva with oral and nasopharyngeal swabs for SARS-CoV-2 detection on various commercial and laboratory-developed assays.
The accurate laboratory detection of the severe acute respiratory syndrome coronavirus 2 (SARSâCoVâ2) is a crucial element in the fight against coronavirus disease 2019 (COVIDâ19). Reverse transcriptionâpolymerase chain reaction testing on combined oral and nasopharyngeal swab (ONPS) suffers from several limitations, including the need for qualified personnel, the discomfort caused by ... More
Amyloid Beta Oligomers Activate Death Receptors and Mitochondria-Mediated Apoptotic Pathways in Cerebral Vascular Smooth Muscle Cells; Protective Effects of Carbonic Anhydrase Inhibitors.
AuthorsAnzovino A,Canepa E,Alves M,Lemon NL,Carare RO,Fossati S
JournalCells
PubMed ID38132159
Amyloid beta (Aβ) deposition within the brain vasculature is an early hallmark of Alzheimerâs disease (AD), which triggers loss of brain vascular smooth muscle cells (BVSMCs) in cerebral arteries, via poorly understood mechanisms, altering cerebral blood flow, brain waste clearance, and promoting cognitive impairment. We have previously shown that, in ... More
Temperature Fluctuations Modulate Molecular Mechanisms in Skeletal Muscle and Influence Growth Potential in Beef Steers.
Our investigation elucidated the effects of severe temperature fluctuations on cellular and physiological responses in beef cattle. Eighteen Red Angus beef steers with an average body weight of 351â ±â 24.5 kg were divided into three treatment groups: 1) Control (CON), exposed to a temperature-humidity index (THI) of 42 for 6 h ... More
Distribution of phenanthrene in the ospho2 reveals the involvement of phosphate on phenanthrene translocation and accumulation in rice.
AuthorsWang X,Jain A,Cui M,Hu S,Zhao G,Cao Y,Hu F
JournalEcotoxicology and environmental safety
PubMed ID35636234
Human amniotic fluid mesenchymal stem cells attenuate pancreatic cancer cell proliferation and tumor growth in an orthotopic xenograft mouse model.
AuthorsChen YC,Lan YW,Huang SM,Yen CC,Chen W,Wu WJ,Staniczek T,Chong KY,Chen CM
JournalStem cell research & therapy
PubMed ID35659367
BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) is a malignant cancer and chemotherapy ineffectively treats PDAC, leading to the requirement for alternative tumor-targeted treatment. Human amniotic fluid mesenchymal stem cells (hAFMSCs) have been revealed to suppress tumor growth in various cancers and they are a strong candidate for treating PDAC. METHODS: To ... More
MicroRNA-mediated attenuation of branched-chain amino acid catabolism promotes ferroptosis in chronic kidney disease.
AuthorsSone H,Lee TJ,Lee BR,Heo D,Oh S,Kwon SH
JournalNature communications
PubMed ID38016961
Chronic kidney disease can develop from kidney injury incident to chemotherapy with cisplatin, which complicates the prognosis of cancer patients. MicroRNAs regulate gene expression by pairing with specific sets of messenger RNAs. Therefore, elucidating direct physical interactions between microRNAs and their target messenger RNAs can help decipher crucial biological processes ... More
8-Oxoguanine DNA Glycosylase 1 Upregulation as a Risk Factor for Obesity and Colorectal Cancer.
JournalInternational journal of molecular sciences
PubMed ID36982562
DNA damage has been extensively studied as a potentially helpful tool in assessing and preventing cancer, having been widely associated with the deregulation of DNA damage repair (DDR) genes and with an increased risk of cancer. Adipose tissue and tumoral cells engage in a reciprocal interaction to establish an inflammatory ... More
Preclinical Animal Study and Pilot Clinical Trial of Using Enriched Peripheral Blood-Derived Mononuclear Cells for Intervertebral Disc Degeneration.
Low back pain (LBP) is a leading cause of long-term disability globally. Intervertebral disk degeneration (IVDD) is mainly responsible for discogenic pain in LBP-affected young patients. There is no effective therapy to reverse disease severity and IVDD progression. This study investigates the effect of human peripheral blood-derived mononuclear cells (PBMCs) ... More
Microbiota-derived tryptophan metabolites indole-3-lactic acid is associated with intestinal ischemia/reperfusion injury via positive regulation of YAP and Nrf2.
BACKGROUND: Lactobacillus has been demonstrated to serve a protective role in intestinal injury. However, the relationship between Lactobacillus murinus (L. murinus)-derived tryptophan metabolites and intestinal ischemia/reperfusion (I/R) injury yet to be investigated. This study aimed to evaluate the role of L. murinus-derived tryptophan metabolites in intestinal I/R injury and the ... More
SARS-CoV-2 infection of human lung epithelial cells induces TMPRSS-mediated acute fibrin deposition.
Severe COVID-associated lung injury is a major confounding factor of hospitalizations and death with no effective treatments. Here, we describe a non-classical fibrin clotting mechanism mediated by SARS-CoV-2 infected primary lung but not other susceptible epithelial cells. This infection-induced fibrin formation is observed in all variants of SARS-CoV-2 infections, and ... More
Octreotide ameliorates hepatic ischemia-reperfusion injury through SNHG12/TAF15-mediated Sirt1 stabilization and YAP1 transcription.
Impact of the Nucleic Acid Extraction Method and the RT-qPCR Assay on SARS-CoV-2 Detection in Low-Viral Samples.
AuthorsKomiazyk M,Walory J,Kozinska A,Wasko I,Baraniak A
JournalDiagnostics (Basel, Switzerland)
PubMed ID34943484
COVID-19 was initially reported in China at the end of 2019 and soon thereafter, in March 2020, the WHO declared it a pandemic. Until October 2021, over 240 million COVID-19 cases were recorded, with 4.9 mln deaths. In order to stop the spread of this disease, it is crucial to ... More
Interleukin-1β secretion induced by mucosa-associated gut commensal bacteria promotes intestinal barrier repair.
AuthorsWu WH,Kim M,Chang LC,Assie A,Saldana-Morales FB,Zegarra-Ruiz DF,Norwood K,Samuel BS,Diehl GE
JournalGut microbes
PubMed ID34989321
The gut microbiota is essential for maintenance and repair of the intestinal epithelial barrier. As shifts in both intestinal epithelial barrier function and microbiota composition are found in inflammatory bowel disease patients, it is critical to understand the role of distinct bacteria in regulating barrier repair. We identified a mouse ... More
Phosphorylation of phase-separated p62 bodies by ULK1 activates a redox-independent stress response.
NRF2 is a transcription factor responsible for antioxidant stress responses that is usually regulated in a redoxâdependent manner. p62 bodies formed by liquidâliquid phase separation contain Ser349âphosphorylated p62, which participates in the redoxâindependent activation of NRF2. However, the regulatory mechanism and physiological significance of p62 phosphorylation remain unclear. Here, we ... More
SwabExpress: An end-to-end protocol for extraction-free COVID-19 testing.
BACKGROUND: The urgent need for massively scaled clinical testing for SARS-CoV-2, along with global shortages of critical reagents and supplies, has necessitated development of streamlined laboratory testing protocols. Conventional nucleic acid testing for SARS-CoV-2 involves collection of a clinical specimen with a nasopharyngeal swab in transport medium, nucleic acid extraction, ... More
Intraocular Adeno-Associated Virus-Mediated Transgene Endothelin-1 Delivery to the Rat Eye Induces Functional Changes Indicative of Retinal Ischemia-A Potential Chronic Glaucoma Model.
AuthorsNordahl KML,Fedulov V,Holm A,Haanes KA
JournalCells
PubMed ID37566067
Endothelin-1 (ET-1) overactivity has been implicated as a factor contributing to glaucomatous neuropathy, and it has been utilized in animal models of retinal ischemia. The functional effects of long-term ET-1 exposure and possible compensatory mechanisms have, however, not been investigated. This was therefore the purpose of our study. ET-1 was ... More
Vaccination with SARS-CoV-2 Spike Protein and AS03 Adjuvant Induces Rapid Anamnestic Antibodies in the Lung and Protects Against Virus Challenge in Nonhuman Primates.
Adjuvanted soluble protein vaccines have been used extensively in humans for protection against various viral infections based on their robust induction of antibody responses. Here, soluble prefusion-stabilized spike trimers (preS dTM) from the severe acute respiratory syndrome coronavirus (SARS-CoV-2) were formulated with the adjuvant AS03 and administered twice to nonhuman ... More
Impact of Dietary Modifications on Plasma Sirtuins 1, 3 and 5 in Older Overweight Individuals Undergoing 12-Weeks of Circuit Training.
Sirtuins are nicotinamide adenine dinucleotide (NAD )-dependent deacetylases that regulate numerous pathways such as mitochondrial energy metabolism in the human body. Lower levels of these enzymes were linked to diseases such as diabetes mellitus and were also described as a result of aging. Sirtuins were previously shown to be under ... More
Induction of type II collagen expression in M2 macrophages derived from peripheral blood mononuclear cells.
The human type II collagen (Col II), specifically expressed in chondrocytes, is a crucial component of the adult hyaline cartilage. We examine the potential of artificial induction of Col II in human peripheral blood mononuclear cells (PBMNCs) as a novel Col II provider. Human PBMNCs were purified and were treated ... More
Swab pooling: A new method for large-scale RT-qPCR screening of SARS-CoV-2 avoiding sample dilution.
To minimize sample dilution effect on SARS-CoV-2 pool testing, we assessed analytical and diagnostic performance of a new methodology, namely swab pooling. In this method, swabs are pooled at the time of collection, as opposed to pooling of equal volumes from individually collected samples. Paired analysis of pooled and individual ... More
Mitochondrial Homeostasis in Obesity-related Hypertriglyceridemia.
AuthorsMela V,Ruiz-Limón P,Balongo M,Motahari Rad H,Subiri-Verdugo A,Gonzalez-Jimenez A,Soler R,Ocaña L,El Azzouzi H,Tinahones FJ,Valdivielso P,Murri M
JournalThe Journal of clinical endocrinology and metabolism
PubMed ID35608825
CONTEXT: The prevalence of obesity and hypertriglyceridemia is an alarming worldwide health issue. Mitochondria play a central role in these disorders as they control cell metabolism. OBJECTIVE: The aim of the present study was to characterize mitochondrial homeostasis in subcutaneous and visceral adipose tissue (SAT and VAT) in grade III ... More
The impact of obesity-related raised intracranial pressure in rodents.
AuthorsWestgate CSJ,Hagen SM,Israelsen IME,Hamann S,Jensen RH,Eftekhari S
JournalScientific reports
PubMed ID35650312
Elevated intracranial pressure (ICP) is observed in many brain disorders. Obesity has been linked to ICP pathogenesis in disorders such as idiopathic intracranial pressure (IIH). We investigated the effect of diet induced obesity (DIO) on ICP and clinically relevant sequelae. Rats were fed either a control or high fat diet. ... More
A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states.
Microglia are emerging as key drivers of neurological diseases. However, we lack a systematic understanding of the underlying mechanisms. Here, we present a screening platform to systematically elucidate functional consequences of genetic perturbations in human induced pluripotent stem cell-derived microglia. We developed an efficient 8-day protocol for the generation of ... More
Sphingosine kinase 1 is involved in triglyceride breakdown by maintaining lysosomal integrity in brown adipocytes.
AuthorsMorishige JI,Yoshioka K,Nakata H,Ishimaru K,Nagata N,Tanaka T,Takuwa Y,Ando H
JournalJournal of lipid research
PubMed ID37751791
Sphingosine 1-phosphate (S1P) has been implicated in brown adipose tissue (BAT) formation and energy consumption; however, the mechanistic role of sphingolipids, including S1P, in BAT remains unclear. Here, we showed that, in mice, BAT activation by cold exposure upregulated mRNA and protein expression of the S1P-synthesizing enzyme sphingosine kinase 1 ... More
Skeletal Muscle Spheroids as Building Blocks for Engineered Muscle Tissue.
AuthorsJohnson N,Filler AC,Sethi A,Smith LR,Leach JK
JournalACS biomaterials science & engineering
PubMed ID38113146
[Image: see text] Spheroids exhibit enhanced cellâcell interactions that facilitate improved survival and mimic the physiological cellular environment in vivo. Cell spheroids have been successfully used as building blocks for engineered tissues, yet the viability of this approach with skeletal muscle spheroids is poorly understood, particularly when incorporated into three-dimensional ... More
New-Born Screening for Spinal Muscular Atrophy: Results of a Latvian Pilot Study.
AuthorsGailite L,Sterna O,Konika M,Isakovs A,Isakova J,Micule I,Setlere S,Diriks M,Auzenbaha M
JournalInternational journal of neonatal screening
PubMed ID35225937
New disease-modifying treatments have recently been approved for 5q spinal muscular atrophy (SMA) and early treatment has been associated with a better clinical outcome. Accordingly, new-born screening (NBS) for SMA should be implemented to ensure early diagnosis of affected individuals. The aim of this study was to determine the feasibility ... More
Interdental oral hygiene interventions elicit varying compositional microbiome changes in naturally occurring gingivitis: Secondary data analysis from a clinical trial.
AuthorsGe Y,Bamashmous S,Mancinelli-Lyle D,Zadeh M,Mohamadzadeh M,Kotsakis GA
JournalJournal of clinical periodontology
PubMed ID38088457
A male steroid controls female sexual behaviour in the malaria mosquito.
Insects, unlike vertebrates, are widely believed to lack male-biased sex steroid hormones(1). In the malaria mosquito Anopheles gambiae, the ecdysteroid 20-hydroxyecdysone (20E) appears to have evolved to both control egg development when synthesized by females(2) and to induce mating refractoriness when sexually transferred by males(3). Because egg development and mating ... More
Indole-3-Propionic Acid, a Gut-Derived Tryptophan Metabolite, Associates with Hepatic Fibrosis.
Background and Aims: Gut microbiota-derived metabolites play a vital role in maintenance of human health and progression of disorders, including obesity and type 2 diabetes (T2D). Indole-3-propionic acid (IPA), a gut-derived tryptophan metabolite, has been recently shown to be lower in individuals with obesity and T2D. IPAâs beneficial effect on ... More
NcRNAs-mediated P2RX1 expression correlates with clinical outcomes and immune infiltration in patients with breast invasive carcinoma.
AuthorsXu Y,Zou B,Fan B,Li B,Yu J,Wang L,Zhang J
JournalAging
PubMed ID35585027
The development of novel treatments for breast invasive carcinoma (BC) has been stagnant. P2RX1, a member of the purinergic receptor family, has been found to have a prognostic impact in several tumors. Therefore, we analyzed the expression pattern of P2RX1 in pan-cancers including BC and its impact on survival and ... More
Viral Etiological Agent(s) of Respiratory Tract Infections in Symptomatic Individuals during the Second Wave of COVID-19 Pandemic: A Single Drive-Thru Mobile Collection Site Study.
AuthorsKozinska A,Wegrzynska K,Komiazyk M,Walory J,Wasko I,Baraniak A
JournalPathogens (Basel, Switzerland)
PubMed ID35456150
One of the tools to contain the SARS-CoV-2 pandemic was to increase the number of performed tests and to improve the access to diagnostics. To this effect, mobile collection sites (MCSs) were established. This study was performed on samples collected at the MCS between November 2020 and March 2021. We ... More
Sublethal enteroviral infection exacerbates disease progression in an ALS mouse model.
AuthorsXue YC,Liu H,Mohamud Y,Bahreyni A,Zhang J,Cashman NR,Luo H
JournalJournal of neuroinflammation
PubMed ID35022041
BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease of the motor neuron system associated with both genetic and environmental risk factors. Infection with enteroviruses, including poliovirus and coxsackievirus, such as coxsackievirus B3 (CVB3), has been proposed as a possible causal/risk factor for ALS due to the evidence that ... More
Heat shock protein 27 regulates myogenic and self-renewal potential of bovine satellite cells under heat stress.
AuthorsKim WS,Daddam JR,Keng BH,Kim J,Kim J
JournalJournal of animal science
PubMed ID37688555
While satellite cells play a key role in the hypertrophy, repair, and regeneration of skeletal muscles, their response to heat exposure remains poorly understood, particularly in beef cattle. This study aimed to investigate the changes in the transcriptome, proteome, and proliferation capability of bovine satellite cells in response to different ... More
PMMA Microcapsules for the Inactivation of SARS-CoV-2.
AuthorsSousa VI,Parente JF,Marques JF,Calçada C,Veiga MI,Osório NS,Tavares CJ
JournalACS omega
PubMed ID35785261
[Image: see text] Surface disinfection currently plays a decisive role in the epidemiological situation caused by the SARS-CoV-2 coronavirus. However, most disinfection products available on the market have a high evaporation rate and only an immediate action and not continuous, creating the need for a high frequency of disinfection. To ... More
Differential parathyroid and kidney Ca(2+)-sensing receptor activation in autosomal dominant hypocalcemia 1.
Authorsvan Megen WH,Tan RSG,Alexander RT,Dimke H
JournalEBioMedicine
PubMed ID35313217
BACKGROUND: Parathyroid Ca(2+)-sensing receptor (CaSR) activation inhibits parathyroid hormone (PTH) release, while activation of renal CaSRs attenuates Ca(2+) transport and increases expression of the pore-blocking claudin-14. Patients with autosomal dominant hypocalcemia 1 (ADH1), due to activating CASR mutations, exhibit hypocalcemia but not always hypercalciuria (elevated Ca(2+) in urine). The latter ... More