Thermo Scientific GPCR Extraction and Stabilization Reagent enables efficient extraction and stabilization of G-coupled protein receptors (GPCRs) and other membrane-associated proteins from cultured mammalian cells or tissue while preserving longer-term receptor integrity.
Features of GPCR Extraction and Stabilization Reagent include: • Ease-of-use—all-in-one formulation to extract and stabilize GPCRs in one hour • GPCR preservation—stabilizes the receptor in a detergent micelle to ensure receptor function • Optimized—formulation and procedure provide gentle but efficient extraction of GPCRs from the plasma membrane • Compatible—lysate is directly compatible with downstream applications including western blotting, immunoprecipitation, and receptor ligand binding assays
GPCR Extraction and Stabilization Reagent is simple to use. The sample is first harvested, washed, and pelleted. Receptor is then extracted using the reagent, followed by centrifugation. The resulting whole cell lysate contains solubilized and stabilized receptors. The reagent has been tested for use with endogenous and overexpressed GPCRs, and receptor function has been verified by receptor ligand binding assays.
GPCRs are a large family of receptors with seven transmembrane domains that play a key role in transmitting signals across the cell membrane. They are effective drug targets for genetic and immune system disorders and serve as potential biomarkers and therapeutic targets for cancer treatment. One of the major challenges in GPCR research is the ability to efficiently extract the receptor from the membrane without compromising the structural and functional integrity of the protein. GPCR Extraction and Stabilization Reagent encapsulates the receptor in a detergent micelle optimized to ensure receptor function for up to one week when stored at 4°C and one month when stored at -20°C post extraction, providing more flexibility for receptor analysis.
References 1. Nieto Gutierrez A, McDonald PH. GPCRs: Emerging anti-cancer drug targets. Cell Signal. 2018 Jan;41:65-74. 2. Zhang S, Togo S, Minakata K, Gu T, Ohashi R, Tajima K, Murakami A, Iwakami S, Zhang J, Xie C, Takahashi K. Distinct roles of cholinergic receptors in small cell lung cancer cells. Anticancer Res. 2010 Jan;30(1):97-106.
For Research Use Only. Not for use in diagnostic procedures.
Contents & Storage
Store in refrigerator (2–8°C).
Preserves functionality of overexpressed M3 receptor
Preserves functionality of receptors in cells and tissue
Compatible with immunoprecipitation
Preparation of lysates with soluble and stable GPCRs
Efficient solubilization of GPCRs from cells and tissues