The SureTect System is an optimized solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.
The Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay includes:
Enriched samples are pipetted into pre-filled lysis tubes, along with Proteinase K, before incubation, to lyse any bacterial cells present in the sample and release their DNA into the solution. The lysate is then loaded into the SureTect Escherichia coli O157:H7 and STEC Screening PCR tube to re-hydrate the pre-loaded PCR pellet which contains all the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal amplification control (IAC). The PCR tubes are then sealed, loaded into Applied Biosystems™ QuantStudio™ 5 Food Safety Real Time PCR instrument with RapidFinder™ Analysis Software v1.1 or later. After completing the run, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required. The internal amplification control confirms correct assay conditions have occurred.
As with all test methods, the source, formulation and quality of enrichment medium can influence the results. Thermo Fisher Scientific has evaluated the SureTect PCR Assays workflows with industry leading Thermo Scientific™ Oxoid™ and Thermo Scientific™ Remel™ media formulations, as well as, ISO formulations where fit for purpose.
Not all products are available for sale in all territories. Please inquire.