Thermo Scientific™

Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL

Catalog number: A44301
Have Questions?
Thermo Scientific™

Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL

Catalog number: A44301
Have Questions?
Quickly remove non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with a 7K MWCO in fewer than 15 minutes with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.
 
Catalog Number
A44301
Unit Size
25 columns
Description
Zeba™ Dye and Biotin Removal Spin Columns, 5 mL
Quantity
25 Columns
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 5 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity25 Columns
Sample Volume (Metric)1 to 2 mL
Unit Size25 columns
Catalog Number
Unit Size
5 columns
Description
Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Quantity
5 Columns
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity5 Columns
Sample Volume (Metric)50 to 120 μL
Unit Size5 columns
Catalog Number
Unit Size
25 columns
Description
Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Quantity
25 Columns
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity25 Columns
Sample Volume (Metric)50 to 120 μL
Unit Size25 columns
Catalog Number
Unit Size
2 x 25 columns
Description
Zeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Quantity
2 x 25 Columns
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity2 x 25 Columns
Sample Volume (Metric)50 to 120 μL
Unit Size2 x 25 columns
Catalog Number
Unit Size
5 columns
Description
Zeba™ Dye and Biotin Removal Spin Columns, 2 mL
Quantity
5 Columns
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 2 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity5 Columns
Sample Volume (Metric)400 to 700 μL
Unit Size5 columns
Catalog Number
Unit Size
25 columns
Description
Zeba™ Dye and Biotin Removal Spin Columns, 2 mL
Quantity
25 Columns
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 2 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity25 Columns
Sample Volume (Metric)400 to 700 μL
Unit Size25 columns
Catalog Number
Unit Size
5 columns
Description
Zeba™ Dye and Biotin Removal Spin Columns, 5 mL
Quantity
5 Columns
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 5 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity5 Columns
Sample Volume (Metric)1 to 2 mL
Unit Size5 columns
Catalog Number
Unit Size
5 columns
Description
Zeba™ Dye and Biotin Removal Spin Columns, 10 mL
Quantity
5 Columns
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 10 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity5 Columns
Sample Volume (Metric)2 to 4 mL
Unit Size5 columns
Catalog Number
Unit Size
5 x 5 columns
Description
Zeba™ Dye and Biotin Removal Spin Columns, 10 mL
Quantity
5 x 5 Columns
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 10 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity5 x 5 Columns
Sample Volume (Metric)2 to 4 mL
Unit Size5 x 5 columns
Catalog Number
Unit Size
2 plates
Description
Zeba™ Dye and Biotin Removal Filter Plates, 96-well
Quantity
2 Plates
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionZeba™ Dye and Biotin Removal Filter Plates, 96-well
Shipping ConditionRoom Temperature
Format96-well Filter Plate
Column TypeProprietary Resin
Product LineZeba™
Quantity2 Plates
Sample Volume (Metric)40 to 100 μL
Unit Size2 plates
Showing 10 of 11Show more
Catalog NumberSpecificationsUnit SizeDescriptionQuantityPrice (USD)AvailabilityQuantity
A44301Full specifications
25 columnsZeba™ Dye and Biotin Removal Spin Columns, 5 mL25 Columns
-
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 5 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity25 Columns
Sample Volume (Metric)1 to 2 mL
Unit Size25 columns
A44296SFull specifications
5 columnsZeba™ Dye and Biotin Removal Spin Columns, 0.5 mL5 Columns
-
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity5 Columns
Sample Volume (Metric)50 to 120 μL
Unit Size5 columns
A44296Full specifications
25 columnsZeba™ Dye and Biotin Removal Spin Columns, 0.5 mL25 Columns
-
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity25 Columns
Sample Volume (Metric)50 to 120 μL
Unit Size25 columns
A44297Full specifications
2 x 25 columnsZeba™ Dye and Biotin Removal Spin Columns, 0.5 mL2 x 25 Columns
-
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 0.5 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity2 x 25 Columns
Sample Volume (Metric)50 to 120 μL
Unit Size2 x 25 columns
A44298Full specifications
5 columnsZeba™ Dye and Biotin Removal Spin Columns, 2 mL5 Columns
-
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 2 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity5 Columns
Sample Volume (Metric)400 to 700 μL
Unit Size5 columns
A44299Full specifications
25 columnsZeba™ Dye and Biotin Removal Spin Columns, 2 mL25 Columns
-
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 2 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity25 Columns
Sample Volume (Metric)400 to 700 μL
Unit Size25 columns
A44300Full specifications
5 columnsZeba™ Dye and Biotin Removal Spin Columns, 5 mL5 Columns
-
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 5 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity5 Columns
Sample Volume (Metric)1 to 2 mL
Unit Size5 columns
A44302Full specifications
5 columnsZeba™ Dye and Biotin Removal Spin Columns, 10 mL5 Columns
-
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 10 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity5 Columns
Sample Volume (Metric)2 to 4 mL
Unit Size5 columns
A44303Full specifications
5 x 5 columnsZeba™ Dye and Biotin Removal Spin Columns, 10 mL5 x 5 Columns
-
DescriptionZeba™ Dye and Biotin Removal Spin Columns, 10 mL
Shipping ConditionRoom Temperature
FormatSpin Column
Column TypeProprietary Resin
Product LineZeba™
Quantity5 x 5 Columns
Sample Volume (Metric)2 to 4 mL
Unit Size5 x 5 columns
A44304Full specifications
2 platesZeba™ Dye and Biotin Removal Filter Plates, 96-well2 Plates
-
DescriptionZeba™ Dye and Biotin Removal Filter Plates, 96-well
Shipping ConditionRoom Temperature
Format96-well Filter Plate
Column TypeProprietary Resin
Product LineZeba™
Quantity2 Plates
Sample Volume (Metric)40 to 100 μL
Unit Size2 plates
Showing 10 of 11Show more

Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. Pierce Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. When using the appropriate amount of sample and working within buffer conditions, these columns and filter plates can successfully remove almost any fluorescent dye. Even dilute protein samples (25 μg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). These products are not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns and filter plates include:
Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
High recovery—low-binding resin maximizes protein recovery
Easy-to-use—no cumbersome column preparation or equilibration
Fast—small-molecule removal and protein recovery in less than 15 minutes
Flexible—available in spin columns and filter spin plates for a range of needs

Sample capacities
• 0.5-mL spin column: 50 to 120 μL sample
• 2-mL spin column: 400 to 700 μL sample
• 5-mL spin column: 1 to 2 mL sample
• 96-well filter plate: 40 to 100 μL sample (per well)

Available formats
Spin columns: 0.5, 2, 5, and 10 mL
96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store in refrigerator (2–8°C).

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