MycoSEQ Assay Service is a fast and cost-effective way to verify the absence of mycoplasma in your cell lines. This service leverages the MycoSEQ Mycoplasma Detection Kit, a real-time PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7. The primers were designed using a proprietary bioinformatics pipeline to detect >90 mycoplasma species with high specificity and no cross-reactivity to closely related bacterial species.

The MycoSEQ Assay Service offers:

• Fast data delivery – turnaround time of four weeks or less
• Convenient sample prep – just send a frozen cell pellet
• High sensitivity – demonstrated by internal and external validations to detect 10 cfu/mL
• High specificity – includes proprietary Discriminatory Positive Control to differentiate true positive from any potential cross-contamination positives
• Exceptional support – a dedicated project manager for frequent project communication

How the MycoSEQ Assay Service works:

• You send a pellet with two million cryopreserved cells to our laboratory for processing.
• We prepare a DNA template with the PrepSEQ Mycoplasma Nucleic Acid Extraction Kit.
• We process the samples on the QuantStudio 12K Flex Real-Time PCR System.
• We analyze your data with AccuSEQ Real-Time Detection Software.

What you get

You will receive a comprehensive report that includes a data table confirming the results for your samples alongside results for the positive and negative assay controls.