Thermo Scientific™

SureTect™ Escherichia coli STEC Identification PCR Assay

Rapidly detect multiple genetic targets for Shiga-toxin producing Escherichia coli O157:H7 and other STEC in food and environmental samples with the Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC PCR workflow. The Thermo Scientific™ SureTect™ Escherichia coli STEC Identification PCR Assay is part of a complete workflow for the rapid detection of Escherichia coli O157:H7, stx, eae, and the Escherichia coli STEC serotypes (O26, O45, O103, O111, O121, O145) from food samples. The assay incorporates an optimized liquid lysis step, providing a quick and simple method for preparing the sample for rapid detection on the Applied Applied Biosystems™ QuantStudio™ 5 Food Safety Real Time PCR System and software. Samples assessed as positive using the SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay are further tested using this SureTect™ Escherichia coli STEC Identification PCR Assay.The assay has been extensively tested on a range of food matrices from food manufacturing environments, including raw meat (excluding poultry), vegetables, fruits, juices, and dairy samples and enables users to reliably detect and differentiate Shiga-toxin producing Escherichia coli O157:H7 and other STEC in as little as 10 hours.
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The SureTect System is an optimized solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.

Simple

  • Easy-to-use with single enrichment step and pre-dispensed reagents.
  • Detect multiple targets in the same run with universal PCR protocols across the range.

Fast

  • Optimized workflow delivers on-screen results in as little as 24 hours
  • Maximum flexibility for operational efficiency with the ability to run all assays simultaneously and to test up to 96 samples in each run.

Smart

  • Intuitive, user-friendly software delivers reliable results, with no subjective interpretation.
  • Actionable data supports fast decisions in a complex product supply chain.

The Thermo Scientific™ SureTect™ Escherichia coli STEC Identification PCR Assay includes:

  • SureTect Escherichia coli STEC Identification PCR Tubes
  • SureTect PCR Caps

The lysate of screened positive samples is pipetted into the SureTect Escherichia coli STEC Identification PCR tube to re-hydrate the pre-loaded PCR pellet which contains all the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal amplification control (IAC). The PCR tubes are then sealed, loaded into Applied Biosystems™ QuantStudio™ 5 Food Safety Real Time PCR instrument with RapidFinder™ Analysis Software v1.1 or later. After completing the run, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required. The internal amplification control confirms correct assay conditions have occurred.

As with all test methods, the source, formulation and quality of enrichment medium can influence the results. Thermo Fisher Scientific has evaluated the SureTect PCR Assays workflows with industry leading Thermo Scientific™ Oxoid™ and Thermo Scientific™ Remel™ media formulations, as well as, ISO formulations where fit for purpose.

Not all products are available for sale in all territories. Please inquire.

For Testing of Food and Environmental Samples Only.