The SureTect System is an optimized solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.
The Thermo Scientific™ SureTect™ Escherichia coli STEC Identification PCR Assay includes:
The lysate of screened positive samples is pipetted into the SureTect Escherichia coli STEC Identification PCR tube to re-hydrate the pre-loaded PCR pellet which contains all the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal amplification control (IAC). The PCR tubes are then sealed, loaded into Applied Biosystems™ QuantStudio™ 5 Food Safety Real Time PCR instrument with RapidFinder™ Analysis Software v1.1 or later. After completing the run, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required. The internal amplification control confirms correct assay conditions have occurred.
As with all test methods, the source, formulation and quality of enrichment medium can influence the results. Thermo Fisher Scientific has evaluated the SureTect PCR Assays workflows with industry leading Thermo Scientific™ Oxoid™ and Thermo Scientific™ Remel™ media formulations, as well as, ISO formulations where fit for purpose.
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