Search
Search
View additional product information for MagMAX™ Cell and Tissue DNA Extraction Buffer - FAQs (A45469, A45470)
3 product FAQs found
Yes, we have protocols for using the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) to isolate genomic DNA from these sample types, but they require purchase of an additional Cell and Tissue Extraction Buffer. The kit and buffer can be purchased together as Cat. No. A45721 or the Cell and Tissue Extraction Buffer can be purchased as a stand alone item (Cat. No. A45469 or A45470).
The suggested workflow for bone marrow aspirates is as follows:
- Use only up to 200 µL of bone marrow aspirate
- Centrifuge at 200 x g for 10 mins
- Remove the supernatant (leaving about 50 µL of aspirate with the loose pellet) and resuspend the cell pellet in 400 µL of Cell and Tissue Extraction Buffer
- From this point, follow the protocol for isolation of DNA from buffy coat samples
The suggested workflow for 1-2.0 x 10E6 PBMCs is as follows:
- Centrifuge at 500-800 x g for 10 mins
- Remove the supernatant (leaving the cell pellet) an resuspend the cell pellet in 400 L of Cell and Tissue Extraction Buffer
- From this point, follow the protocol for isolation of DNA from cultured cell samples
Yes, we have protocols for using the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) to isolate DNA from cells and tissue, but they require purchase of an additional Cell and Tissue Extraction Buffer. The kit and buffer can be purchased together as Cat. No. A45721 or the Cell and Tissue Extraction Buffer can be purchased as a stand alone item (Cat. No. A45469 or A45470).
RNase A digestion is optional, but we highly recommend doing it. We recommend using PureLink RNase A (20 mg/mL) (Cat. No. 12091021).