Applied Biosystems™

resDNASEQ™ Quantitative HEK293 DNA Kits

Catalog number: A46014
Applied Biosystems™

resDNASEQ™ Quantitative HEK293 DNA Kits

Catalog number: A46014
The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines used in the development of gene therapy, cell-based vaccines, and similar biotherapeutics. Reliable and rapid, the resDNASEQ system enables sensitive and specific quantitation of HEK293 cell DNA. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples with different sample matrices to purified final product.
 
Catalog Number
A46014
Unit Size
100 reactions
Includes
Assay Kit only
Price (USD)
Price: 3,360.00
Your price:
Availability
-
Full specifications
Quantity
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellHEK
Label or DyeFAM
Product LineresDNASEQ™
Shipping ConditionDry Ice
No. of Reactions100 Reactions
IncludesAssay Kit only
Unit Size100 reactions
Catalog Number
Unit Size
100 reactions
Includes
Assay Kit and Sample Preparation
Price (USD)
Price: 3,745.00
Your price:
Availability
-
Full specifications
Quantity
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellHEK
Label or DyeFAM
Product LineresDNASEQ™
No. of Reactions100 Reactions
IncludesAssay Kit and Sample Preparation
Unit Size100 reactions
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeIncludesPrice (USD)AvailabilityQuantity
A46014Full specifications
100 reactionsAssay Kit only
Price: 3,360.00
Your price:
-
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellHEK
Label or DyeFAM
Product LineresDNASEQ™
Shipping ConditionDry Ice
No. of Reactions100 Reactions
IncludesAssay Kit only
Unit Size100 reactions
A46565Full specifications
100 reactionsAssay Kit and Sample Preparation
Price: 3,745.00
Your price:
-
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellHEK
Label or DyeFAM
Product LineresDNASEQ™
No. of Reactions100 Reactions
IncludesAssay Kit and Sample Preparation
Unit Size100 reactions
Showing 2 of 2
Features of the resDNASEQ Quantitative HEK293 DNA Kit include:
• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan primer/probe mix, and HEK293 genomic DNA standard

This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.

Integrated workflow solution
The resDNASEQ Quantitative HEK293 DNA Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing. The kit is compatible with the PrepSEQ Residual DNA Sample Preparation Kit, which offers both a manual and automated sample preparation workflow. The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems QuantStudio 5 Real-Time PCR System. Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.

Easy to use
With the resDNASEQ Quantitative HEK293 DNA Kit, you perform qPCR analysis to compare the amount of HEK293 DNA in your test samples to a standard curve generated with known amounts of purified HEK293 DNA standard. The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to HEK293 genomic DNA standard, as well as step-by-step instructions.

Consistent performance
For accurate quantitation of residual HEK293 DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.

*Due to the high degree of similarity between genomes, DNA from closely-related primate species, such as monkey (Vero), chimpanzee, and orangutan, are expected be detected by the resDNASEQ HEK293 Quantitation Kit.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

resDNASEQ HEK293 DNA Control
• HEK293 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C
• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use

resDNASEQ HEK293 DNA Real-Time PCR Reagents
• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
• HEK293 DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use

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