Applied Biosystems™

resDNASEQ™ Quantitative HEK293 DNA Kit

Catalog number: A46014
Applied Biosystems™

resDNASEQ™ Quantitative HEK293 DNA Kit

Catalog number: A46014
Catalog Number
Unit Size
100 reactions
Price (USD)
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A46014100 reactions
Your Price:
Product Overview
Citations & References
Additional Information
The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines used in the development of gene therapy, cell-based vaccine, and similar biotherapeutics. Reliable and rapid, the resDNASEQ system enables sensitive and specific quantitation of HEK293 cell DNA*. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples with different sample matrices to purified final product—whether the sample contains high molecular weight or sheared DNA.

• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, TaqMan primer/probe mix, and HEK293 genomic DNA standard

Integrated workflow solution
The resDNASEQ Quantitative HEK293 DNA Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing. The kit is compatible with the PrepSEQ Residual DNA Sample Preparation Kit, which offers both a manual and automated sample preparation workflow. The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems QuantStudio 5 Real-Time PCR System. Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.

This kit does not include sample preparation reagents—for this, please see Cat. No. A46565.

Easy to use
With the resDNASEQ Quantitative HEK293 DNA Kit, you perform qPCR analysis to compare the amount of HEK293 DNA in your test samples to a standard curve generated with known amounts of purified HEK293 DNA standard. The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to HEK293 genomic DNA standard, as well as step-by-step instructions.

Consistent performance
For accurate quantitation of residual HEK293 DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.

*Due to the high degree of similarity between genomes, DNA from closely-related primate species, such as monkey (Vero), chimpanzee, and orangutan, are expected be detected by the resDNASEQ HEK293 Quantitation Kit.


Detection Method
GC-Rich PCR Performance
PCR Method
Reaction Speed
Host Cell
Product Line
Product Type
Quantitative DNA Kit
Shipping Condition
Dry Ice

Contents & Storage

resDNASEQ HEK293 DNA Control
• HEK293 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C
• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first use

resDNASEQ HEK293 DNA Real-Time PCR Reagents
• 2X Environmental Master Mix, 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
• 10X HEK293 DNA Assay Mix, 300 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use