Ion Torrent™

Ion AmpliSeq™ Microbiome Health Research Kit, Ion 540 bundle

Catalog number: A46496
Ion Torrent™

Ion AmpliSeq™ Microbiome Health Research Kit, Ion 540 bundle

Catalog number: A46496
Catalog Number
A46496
Unit Size
256 samples
Price (USD)
Catalog Number
Unit Size
384 samples
Price (USD)
Catalog Number
Unit Size
48 samples
Price (USD)
Catalog NumberUnit SizePrice (USD)
A46496256 samples
A46497384 samples
A4649548 samples
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Recommendations

This product is available in different configurations, ranging from 48 up to 384 samples per kit to accommodate your Microbiome research needs. The product bundles provide reagents and materials for a cost-effective and comprehensive, targeted next-generation sequencing (NGS) workflow for highly sensitive, specific, and robust characterization of human gut microbiome from stool samples, with a full analysis solution providing optimized informatics and visualization.

The Ion AmpliSeq Microbiome Health Research Kit is a targeted NGS assay for characterizing the human gut microbiome, which has been implicated in human disease. The assay consists of two pools of Ion AmpliSeq oligonucleotide primers and associated reagents to generate amplicon libraries for NGS on Ion Torrent platforms. The assay targets multiple hypervariable regions in the 16S rRNA gene, as well as 73 species-specific sequences. With this kit, DNA isolated from human stool samples is used to generate libraries for templating and sequencing on the Ion S5 and Ion Chef systems.

Features of the Ion AmpliSeq Microbiome Health Research Kit include:
• Simple—full sample-to-answer solution consisting of an easy and robust workflow including analysis software
• Robust—enables profiling of the human gut microbiome with excellent sensitivity, specificity, and reproducibility
• Comprehensive—enables accurate taxonomic profiling and relative abundance estimation targeting eight of nine 16S hypervariable regions of 16S rRNA gene and accurate detection of 73 key bacterial species associated with human disease
• Efficient—enables screening of 32 samples multiplexed on a single Ion 540 Chip or 48 samples on a single In 550 Chip, with entire workflow typically completed in three days, including analysis
• Sensitive—compatible with as little as 1 ng input DNA for 16S rRNA Gene Pool and 10 ng for Target Species Pool

This assay is designed to assist researchers in understanding the impact of gut microbiome diversity and composition on human health and disease. The kit enables global characterization of the microbiome by assaying multiple hypervariable regions in the 16S rRNA gene and detection of 73 key bacterial species associated with human diseases, such as cancer, including bacteria linked to potential efficacy of immune-modulatory drugs, gastrointestinal and immunological disorders, as well as infectious disease. We have designed species-specific primers that provide high strain coverage and high specificity performance using the best-in-class amplicon technology. With this approach, reads generated from sequencing can be analyzed to report taxonomic classifications and relative abundance for organisms in the sample with high specificity and sensitivity.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Next Generation Sequencing
For Use With (Application)
Sequencing
No. of Samples
48, 256 or 384
Product Line
Ion AmpliSeq™
Product Type
Microbiome Health Research Kit
Shipping Condition
Dry Ice
Species
Human
Format
Kit

Contents & Storage

• Ion AmpliSeq Microbiome Health Research Panel, store at -5 to -30°C
• Ion AmpliSeq Library Kit Plus (96 reactions), store at -5 to -30°C
• IonCode Barcode Adapters 0101–0196, store at -5 to -30°C

Catalog number A46496 also include:
• Ion 540 Chip Kit, store at room temperature
• Ion S5 Chef Solutions, store at room temperature
• Ion S5 Chef Supplies, store at room temperature

Catalog number A46497 also include:
• Ion 550 Chef Reagents, store at -5 to -30°C
• Ion S5 Sequencing Solutions, store at room temperature
• Ion S5 Sequencing Reagents, store at -5 to -30°C

Figures

Frequently asked questions (FAQs)

Citations & References