RNA-binding proteins (RBPs) are a set of proteins involved in many steps of post-transcriptional regulation to maintain cellular homeostasis. Ovarian cancer (OC) is the most deadly gynecological cancer, but the roles of RBPs in OC are not fully understood. Here, we reported that the RBP QKI5 was significantly negatively correlated ... More
Impact of Non-Saccharomyces Wine Yeast Strains on Improving Healthy Characteristics and the Sensory Profile of Beer in Sequential Fermentation.
AuthorsPostigo V,Sanz P,García M,Arroyo T
JournalFoods (Basel, Switzerland)
PubMed ID35885271
The use of non-Saccharomyces yeasts in brewing is a useful tool for developing new products to meet the growing consumer demand for innovative products. Non-Saccharomyces yeasts can be used both in single and in mixed fermentations with Saccharomyces cerevisiae, as they are able to improve the sensory profile of beers, ... More
Diagnostic accuracy of three commercially available one step RT-PCR assays for the detection of SARS-CoV-2 in resource limited settings.
AuthorsSisay A,Abera A,Dufera B,Endrias T,Tasew G,Tesfaye A,Hartnack S,Beyene D,Desta AF
JournalPloS one
PubMed ID35051204
BACKGROUND: COVID-19 is an ongoing public health pandemic regardless of the countless efforts made by various actors. Quality diagnostic tests are important for early detection and control. Notably, several commercially available one step RT-PCR based assays have been recommended by the WHO. Yet, their analytic and diagnostic performances have not ... More
A Comparative Study of Real-Time RT-PCR-Based SARS-CoV-2 Detection Methods and Its Application to Human-Derived and Surface Swabbed Material.
Real-time RT-PCR remains a gold standard in the detection of various viral diseases. In the coronavirus 2019 pandemic, multiple RT-PCRâbased tests were developed to screen for viral infection. As an emergency response to increasing testing demand, we established a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) PCR diagnostics platform for ... More
Kinesin-14 KIFC1 promotes acrosome formation and chromatin maturation during mouse spermiogenesis.
AuthorsWei YL,Fan XJ,Lin XC,Lin AZ,She ZY,Wang XR
JournalBiochimica et biophysica acta. Molecular cell research
PubMed ID37524262
Vertical transmission and clinical outcome of the neonates born to SARS-CoV-2-positive mothers: a tertiary care hospital-based observational study.
AuthorsKumar P,Fadila,Prasad A,Akhtar A,Chaudhary BK,Tiwari LK,Chaudhry N
JournalBMJ paediatrics open
PubMed ID34697599
BACKGROUND: Neonatal transmission of SARS-CoV-2 from positive mothers to their babies has been a real concern, opening the arena of research in this area. OBJECTIVE: To detect the possibility of vertical transmission of SARS-CoV-2 from COVID-19-positive mothers to their neonates and the clinicopathological outcome in them. DESIGN: A single-centre, prospective, ... More
SARS-CoV-2 R.1 lineage variants that prevailed in Tokyo in March 2021.
The spread of severe acute respiratory syndrome coronavirus 2 (SARSâCoVâ2) variants, such as B.1.1.7 and B.1.351, has become a crucial issue worldwide. Therefore, we began testing all patients with COVIDâ19 for the N501Y and E484K mutations by using polymerase chain reaction (PCR)âbased methods. Nasopharyngeal swab samples from 108 patients who ... More
Viral loads and profile of the patients infected with SARS-CoV-2 Delta, Alpha, or R.1 variants in Tokyo.
The rapid spread of the Delta variant of severe acute respiratory syndrome coronavirus 2 (SARSâCoVâ2) became a serious concern worldwide in summer 2021. We examined the copy number and variant types of all SARSâCoVâ2âpositive patients who visited our hospital from February to August 2021 using polymerase chain reaction (PCR) tests. ... More
Fatal cases after Omicron BA.1 and BA.2 infection: Results of an autopsy study.
JournalInternational journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
PubMed ID36584746
Comparative Performance Evaluation of FilmArray BioFire RP2.1 and MAScIR 2.0 Assays for SARS-CoV-2 Detection.
AuthorsTazi S,Kabbaj H,Zirar J,Zouaki A,El Amin G,El Himeur O,Seffar M
JournalAdvances in virology
PubMed ID35693128
BACKGROUND: RT-PCR is the gold standard for COVID-19 diagnosis, but the lack of standardization of assays, whose diagnostic performance may widely vary, complicates the interpretation of the discrepancies that may be encountered. Study design. We conducted a retrospective study over a ten-month period at the Central Laboratory of Virology of ... More
SR9009 improves heart function after pressure overload independent of cardiac REV-ERB.
The core clock component REV-ERB is essential for heart function. Previous studies show that REV-ERB agonist SR9009 ameliorates heart remodeling in the pressure overload model with transverse aortic constriction (TAC). However, it is unknown whether SR9009 indeed works through cardiac REV-ERB, given that SR9009 might target other proteins and that ... More
MicroRNA-223 Suppresses Human Hepatic Stellate Cell Activation Partly via Regulating the Actin Cytoskeleton and Alleviates Fibrosis in Organoid Models of Liver Injury.
AuthorsAriyachet C,Chuaypen N,Kaewsapsak P,Chantaravisoot N,Jindatip D,Potikanond S,Tangkijvanich P
JournalInternational journal of molecular sciences
PubMed ID36012644
MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate target mRNA expression, and altered expression of miRNAs is associated with liver pathological conditions. Recent studies in animal models have shown neutrophil/myeloid-specific microRNA-223 (miR-223) as a key regulator in the development of various liver diseases including fibrosis, where hepatic stellate cells ... More
Emerging diagnostic tools for detection of COVID-19 and perspective.
AuthorsVerma N,Patel D,Pandya A
JournalBiomedical microdevices
PubMed ID33230627
The COVID-19 caused by a novel coronavirus, named Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) has taken a great toll of life affecting lakhs of people around the globe. It was detected initially in Wuhan, China and has spread rapidly to more than 208 countries to date. A range ... More
Resistance to the isocitrate dehydrogenase 1 mutant inhibitor ivosidenib can be overcome by alternative dimer-interface binding inhibitors.
Ivosidenib, an inhibitor of isocitrate dehydrogenase 1 (IDH1) R132C and R132H variants, is approved for the treatment of acute myeloid leukaemia (AML). Resistance to ivosidenib due to a second site mutation of IDH1 R132C, leading to IDH1 R132C/S280F, has emerged. We describe biochemical, crystallographic, and cellular studies on the IDH1 ... More
High viral loads: what drives fatal cases of COVID-19 in vaccinees? - an autopsy study.
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
PubMed ID35365771
The rate of SARS-CoV-2 infections in vaccinees has become a relevant serious issue. This study aimed to determine the causes of death, histological organ alteration, and viral spread in relation to demographic, clinical-pathological, viral variants, and vaccine types for deceased individuals with proven SARS-CoV-2 infection after vaccination who died between ... More
External quality assessment of HIV-1 DNA quantification assays used in the clinical setting in Italy.
Total cell-associated HIV-1 DNA is a surrogate marker of the HIV-1 reservoir, however, certified systems for its quantification are not available. The Italian HIV DNA Network was launched to validate HIV-1 DNA quantification methods in use at University and Hospital labs. A quality control panel including HIV-1 DNA standards, reconstructed ... More
Serological testing for SARS-CoV-2 in Bosnia and Herzegovina: first report.
INTRODUCTION: Serological detection of SARS-CoV-2-specific immunoglobulins G (IgG) and M (IgM) antibodies is becoming increasingly important in the management of the COVID-19 pandemic. METHODS: We report the first results of COVID-19 serological testing in Bosnia and Herzegovina on 2841 samples collected and analysed in 2 medical institutions in Sarajevo. Antibody ... More
Case of a pregnant woman with probable prolonged SARS-CoV-2 viral shedding 221 days after diagnosis.
AuthorsAkine D,Sasahara T,Koido A,Abe K,Abe K,Oki A,Takeyasu N,Hashimoto I
JournalJournal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy
PubMed ID35367149
We describe a case of probable prolonged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Alpha(B.1.1.7) variant shedding for 221 days from the diagnosis, in a healthy 20-year-old Japanese pregnant woman with a normal delivery. To our knowledge, this is the longest duration of SARS-CoV-2 shedding reported in an immunocompetent individual ... More
Prolonged Unfrozen Storage and Repeated Freeze-Thawing of SARS-CoV-2 Patient Samples Have Minor Effects on SARS-CoV-2 Detectability by RT-PCR.
Reliable transportation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) patient samples from a swabbing station to a diagnostics facility is essential for accurate results. Therefore, cooling or freezing the samples is recommended in case of longer transportation times. In this study, SARS-CoV-2 detectability by RT-PCR was assessed after prolonged ... More
Genome-Wide Identification of Wheat KNOX Gene Family and Functional Characterization of TaKNOX14-D in Plants.
AuthorsLi S,Yao Y,Ye W,Wang S,Zhang C,Liu S,Sun F,Xi Y
JournalInternational journal of molecular sciences
PubMed ID36555558
The KNOX genes play important roles in maintaining SAM and regulating the development of plant leaves. However, the TaKNOX genes in wheat are still not well understood, especially their role in abiotic stress. In this study, a total of 36 KNOX genes were identified, and we demonstrated the function of ... More
Viral load of SARS-CoV-2 Omicron is not high despite its high infectivity.
Patients infected with the Omicron variant of severe acute respiratory syndrome coronavirus 2 has increased worldwide since the beginning of 2022 and the variant has spread more rapidly than the Delta variant, which spread in the summer of 2021. It is important to clarify the cause of the strong transmissibility ... More
Viral mapping in COVID-19 deceased in the Augsburg autopsy series of the first wave: A multiorgan and multimethodological approach.
BACKGROUND: COVID-19 is only partly understood, and the level of evidence available in terms of pathophysiology, epidemiology, therapy, and long-term outcome remains limited. During the early phase of the pandemic, it was necessary to effectively investigate all aspects of this new disease. Autopsy can be a valuable procedure to investigate ... More
Does decreased autophagy and dysregulation of LC3A in astrocytes play a role in major depressive disorder?
Astrocytic dysfunction contributes to the molecular pathogenesis of major depressive disorder (MDD). However, the astrocytic subtype that mainly contributes to MDD etiology and whether dysregulated autophagy in astrocytes is associated with MDD remain unknown. Using a single-nucleus RNA sequencing (snRNA-seq) atlas, three astrocyte subtypes were identified in MDD, while C2 ... More
Bridging a diagnostic Kawasaki disease classifier from a microarray platform to a qRT-PCR assay.
BACKGROUND: Kawasaki disease (KD) is a systemic vasculitis that mainly affects children under 5 years of age. Up to 30% of patients develop coronary artery abnormalities, which are reduced with early treatment. Timely diagnosis of KD is challenging but may become more straightforward with the recent discovery of a whole-blood ... More
Prognostic implications of the ID1 expression in acute myeloid leukemia patients treated in a resource-constrained setting.
INTRODUCTION: The aberrant expression of the inhibitor of DNA binding (ID1) gene has been frequently associated with the leukemogenesis and prognostication acute myeloid leukemia (AML), although its clinical importance has never been investigated in patients treated outside well-controlled clinical trials. METHODS: Using quantitative real-time polymerase chain reaction, we investigated the ... More
Epidermal Growth Factor Receptor T790M Mutation Testing in Non-Small Cell Lung Cancer: An International Collaborative Study to Assess Molecular EGFR T790M Testing in Liquid Biopsy.
AuthorsFilipits M,Kainz V,Sebek V,Zach H,On Behalf Of The Liquid Biopsy Collaborative Study Group
JournalCancers
PubMed ID37444638
SIMPLE SUMMARY: Several liquid biopsy platforms with varying degrees of accuracy are available for EGFR mutation testing in NSCLC. We performed a collaborative study to describe and compare the sensitivity of different liquid biopsy platforms used in clinical routines to detect selected EGFR mutations. In-house PCR-based and NGS-based methods were ... More
RT- PCR testing of upper respiratory tract samples for diagnosis of SARS-CoV-2: Between justification and overestimation, a multi-center international study.
BACKGROUND: It has been found that patients recovered from COVID 19 may still test Reverse Transcriptase- Polymerase Chain Reaction (RT- PCR) positive without being infectious; the reasons are unclear. The occurrence of false-negative results of RT- PCR interferes with a proper diagnosis. The objectives of that work were to determine ... More
First Year of TREC-Based National SCID Screening in Sweden.
JournalInternational journal of neonatal screening
PubMed ID34449549
Screening for severe combined immunodeficiency (SCID) was introduced into the Swedish newborn screening program in August 2019 and here we report the results of the first year. T cell receptor excision circles (TRECs), kappa-deleting element excision circles (KRECs), and actin beta (ACTB) levels were quantitated by multiplex qPCR from dried ... More
Viral load of SARS-CoV-2 Omicron BA.5 is lower than that of BA.2 despite the higher infectivity of BA.5.
BACKGROUND: Sublineage BA.5 of the SARSâCoVâ2 Omicron variant rapidly spread and replaced BA.2 in July 2022 in Tokyo. A high viral load can be a possible cause of high transmissibility. METHODS AND RESULTS: The copy numbers of SARSâCoVâ2 in nasopharyngeal swab samples obtained from all patients visiting the hospital where ... More
Polymerase chain reaction-positivity and predictors for SARS-CoV-2 infection among diagnosed cases' in North West Ethiopia.
AuthorsKebede F,Kebede T
JournalHealth science reports
PubMed ID37900095
BACKGROUND: The lack of sophisticated diagnosing equipment for polymerase chain reaction (PCR) during the incidence of variant types of COVIDâ19 underestimates the morbidity and mortality patterns of this pandemic. Thus, this study aimed to estimate seropositive and confirmatory predictors for COVIDâ19 suspected and tested cases through polymerase chain reaction (RTâPCR) ... More
Genetic Analysis of Dengue Virus in Severe and Non-Severe Cases in Dhaka, Bangladesh, in 2018-2022.
AuthorsRahim R,Hasan A,Phadungsombat J,Hasan N,Ara N,Biswas SM,Nakayama EE,Rahman M,Shioda T
JournalViruses
PubMed ID37243230
Dengue virus (DENV) infections have unpredictable clinical outcomes, ranging from asymptomatic or minor febrile illness to severe and fatal disease. The severity of dengue infection is at least partly related to the replacement of circulating DENV serotypes and/or genotypes. To describe clinical profiles of patients and the viral sequence diversity ... More
Transforming growth factors β and their signaling pathway in renal cell carcinoma and peritumoral space-transcriptome analysis.
JournalClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
PubMed ID38085441
PURPOSE: The aim of the study was to verify hypotheses: Are transforming growth factors TGFβ1-3, their receptors TGFβI-III, and intracellular messenger proteins Smad1-7 involved in the pathogenesis of kidney cancer? What is the expression of genes of the TGFβ/Smads pathway in renal cell carcinoma (RCC) tissues, peritumoral tissues (TME; tumor ... More
Contamination of personal protective equipment during COVID-19 autopsies.
JournalVirchows Archiv : an international journal of pathology
PubMed ID34993593
Confronted with an emerging infectious disease at the beginning of the COVID-19 pandemic, the medical community faced concerns regarding the safety of autopsies on those who died of the disease. This attitude has changed, and autopsies are now recognized as indispensable tools for understanding COVID-19, but the true risk of ... More
THE STUDY OF PROGNOSTIC VALUE OF microRNAs (miR-10b AND -155) AND CDKN2A/P16INK4A IN ORAL SQUAMOUS CELL CARCINOMA.