Invitrogen™

OncoStat™ Panel, SNAP-ChIP™ Spike-in

Catalog number: A47355
Invitrogen™

OncoStat™ Panel, SNAP-ChIP™ Spike-in

Catalog number: A47355
Catalog Number
A47355
Unit Size
100 reactions
Price (USD)
3,005.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
10 reactions
Price (USD)
408.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A47355100 reactions
3,005.00
Your Price:
***
A4734310 reactions
408.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded semi-synthetic nucleosomes bearing distinct histone mutations as spike-in ChIP controls.

• Homogenous, fully defined standards faithfully represent target mononucleosomes in the experimental sample
• Nucleosomes are subjected to rigorous quality control for lot-to-lot consistency
• Unique DNA barcodes can be distinguished from experimental sample genomes
• Spike-ins provide a direct readout of antibody performance in ChIP
• Ability to monitor technical variability between samples
• Defined standards enable universal normalization across experiments

SNAP-ChIP panels are directly compatible with your current ChIP workflow, with semi-synthetic nucleosomes bearing the mutation of interest immunoprecipitated and processed alongside sample chromatin.

Users can directly monitor antibody capability (specificity and enrichment) in a ChIP experiment by measuring the recovery of on- and off-target nucleosomes. The SNAP spike-ins can be further used as a defined standard to normalize your ChIP data, thereby controlling for technical variability across experiments.

The OncoStat Panel consists of a pool of nucleosomes harboring seven well-studied histone H3.3 mutations that have been implicated in cancer (histone H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W and H3.3K36M) plus a wild-type H3.3 control. Further, each of these eight nucleosomes is wrapped by two independent DNA barcodes (16 total barcodes in the panel), allowing for an internal technical replicate in each ChIP reaction. A single spike-in of the panel allows users to check antibody specificity by examining the post-IP recovery of on- versus off-target SNAP-ChIP nucleosomes, supporting the generation of high quality ChIP data.

WARNING: Cancer – www.P65Warnings.ca.gov

Specifications

Description
Recombinant Nucleosome Panel
No. of Tests
100 Reactions
Product Line
OncoStat™ SNAP-ChIP™
Product Type
SNAP-ChIP Panel

Contents & Storage

Store at -5 to -30°C.

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