Invitrogen™

Second Strand cDNA Synthesis Kit

Catalog number:  A48570

Related applications:

cDNA Libraries & Library Construction

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Description

Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features of the Second Strand cDNA Synthesis Kit include:
• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in 1 hour
• Convenient—pre-mixed components
• Complete—includes residual RNA removal reagents

Applications
• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction

WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov

Specifications

Final Product Type: Double Stranded cDNA
For Use With (Application): Cloning
Label or Dye: No
Product Type: Kit
Size: 50 reactions
Reaction Format: Separate Components

Contents & storage

Contents:
• Second Strand Enzyme Mix, 1 mL
• 5X Second Strand Reaction Mix, 250 µL
• 0.5 M EDTA, pH 8.0, 1 mL
• RNase I, 10 U/µL, 500 µL
• Water, nuclease-free, 3 x 1.25 mL

Storage: -15 to -25°C