Second Strand cDNA Synthesis Kit

Catalog number:  A48571

Related applications:

cDNA Libraries & Library Construction

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Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features of the Second Strand cDNA Synthesis Kit include:
• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in 1 hour
• Convenient—pre-mixed components
• Complete—includes residual RNA removal reagents

• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction

WARNING: Cancer and Reproductive Harm –


Final Product Type: Double Stranded cDNA
For Use With (Application): Cloning
Label or Dye: No
Format: Kit
Product Type: Kit
Quantity: 200 Reactions
Size: 200 reactions
Reaction Format: Separate Components

Contents & storage

• Second Strand Enzyme Mix, 4 x 1 mL
• 5X Second Strand Reaction Mix, 4 x 250 µL
• 0.5 M EDTA, pH 8.0, 4 x 1 mL
• RNase I, 10 U/µL, 4 x 500 µL
• Water, nuclease-free, 12 x 1.25 mL

Storage: -15 to -25°C