CTS™ Rotea™ Hi-Flow Single-Use Kit

The Gibco CTS Rotea Hi-Flow Single-Use Kit is part of the Gibco CTS Rotea Counterflow Centrifugation System. The kit isRead more

Catalog Number	Quantity
A49239	5 Kits

Catalog number A49239

Price (MXN)

Quantity:

5 Kits

The Gibco CTS Rotea Hi-Flow Single-Use Kit is part of the Gibco CTS Rotea Counterflow Centrifugation System. The kit is capable of flow rates of 30-160 mL/min. Each kit can perform a wide range of cell processing steps, making ideal for wash & concentrate, separation, isolation, and more. The single-use kit provides flexibility for process development and easily supports commercial scale manufacturing. Each kit is compatible with sterile luer connectors or thermal welding.

Tubing material: DEHP-free PVC
Tube dimensions: 0.160 in. OD, 0.116 in. ID
Pump tube material: Bioprene™ plastic

Kits are gamma-irradiated and individually packaged.

Specifications

Capacity20 L

Cell TypeAgnostic

Certifications/ComplianceISO 13485

DisposableSingle-use

Flow Rate160 mL/min.

For Use With (Application)Cell and Gene Therapy Research, Development, and Manufacturing

MaterialTritan MX711 Copolyester (Chamber), DEHP-free PVC (Tubing)

Max. RCF3000 x g

Quantity5 Kits

Shelf Life18 months from date of manufacture

Sterilization MethodGamma-irradiated

For Use With (Equipment)CTS Rotea Counterflow Centrifugation System

Unit SizeEach

Frequently asked questions (FAQs)

Do you have a calculation formula that allows us to estimate the max feed flow rate/processing time for the CTS Rotea System, depending on the cell diameter?

The Rotea Protocol Builder Software, which includes the Rotea Process Model and Simulator, is available for download from the Rotea website. Common cell types are already pre-loaded into the software, and custom cells can also be created. The CTS Rotea System can separate cells that have at least 4 µm difference in diameter. Thermo Fisher Scientific recommends that customers use protocol optimization and workflow validation testing to meet their process needs. Please visit our webpage (www.thermofisher.com/rotea) to see if the CTS Rotea System will work for your cell type, or submit a request for more information using our demo form (https://www.thermofisher.com/us/en/home/global/forms/life-science/rotea-counterflow-centrifugation-system-cell-therapy.html) to get connected with a Business Development Manager.

Are there plans for incorporating magnetic bead selection in the CTS Rotea System or other ways of automating magnetic bead selection?

Thermo Fisher Scientific strives to provide a catalog of tools to support our diverse customers' needs. If magnetic bead separation using the CTS Rotea System is of interest, Thermo Fisher Scientific recommends that customers use protocol optimization and workflow validation testing to meet their process needs.

How does cell processing with Ficoll medium compare to using the CTS Rotea System?

Ficoll medium has many shortcomings and often does not scale well for cGMP manufacturing. Rotea PBMC isolation protocol takes less time and has been demonstrated to be capable of providing higher cell recovery compared to Ficoll medium. Thermo Fisher Scientific recommends that customers use protocol optimization and workflow validation testing to meet their process needs.

What is the best cryopreservative to use with the CTS Rotea System?

The CTS Rotea single-use kits are manufactured with DEHP-free PVC. Extractables and Leechables testing was conducted with 20% DMSO for up to 15 min. The CTS Rotea System has not been validated for fill-finish applications. Thermo Fisher Scientific recommends that customers use protocol optimization and workflow validation testing to meet their process needs.

Have you performed final formulation on the CTS Rotea System directly into cryopreservation media?

The CTS Rotea System has been demonstrated to be capable of washing cells from cryoprotectant into media and the reverse. The CTS Rotea System has not been validated for fill-finish applications. Thermo Fisher Scientific recommends that customers use protocol optimization and workflow validation testing to meet their process needs

View all CTS™ Rotea™ Hi-Flow Single-Use Kit, 5 Kits FAQs