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AAV-MAX Transfection Kit, 1 L production - FAQs

AAV-MAX Transfection Kit, 1 L production - FAQs

View additional product information for AAV-MAX Transfection Kit - FAQs (A50515, A50516)

8 product FAQs found

In the AAV-MAX Transfection Kit (Cat. No. A50515, A50516), what is the AAV-MAX Enhancer and what does it do?

The AAV-MAX Enhancer increases AAV vector production in Viral Production Cells (VPCs) 2.0. The identity and mechanism of action of the AAV-MAX Enhancer are proprietary.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

In the AAV-MAX Transfection Kit (Cat. No. A50515, A50516), what is the AAV-MAX Transfection Booster and what does it do?

The AAV-MAX Transfection Booster enhances the efficiency of co-transfection of multiple plasmids into high-density Viral Production Cells (VPCs) 2.0. The identity and mechanism of action of the transfection booster are proprietary.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use other transfection reagents, such as PEI (polyethylenimine), in the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Transfection Reagent, AAV-MAX Tranfection Booster, and AAV-MAX Enhancer are specifically designed to work synergistically with the Viral Production Medium and Viral Production Cells 2.0 to provide maximum titers. Mixing and matching of reagents is not recommended, as this will decrease the overall performance.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How scalable is the AAV-MAX transfection protocol?

The AAV-MAX transfection protocol is scalable from 24 deep-well plates to shake flasks to bioreactors. The user guide provides guidance on this.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Are the LV-MAX Enhancer and AAV-MAX Enhancer different?

Yes, these are different, and each is optimized for its intended use.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Will I see a drop in adeno-associated virus (AAV) titer if I don't use the AAV-MAX Enhancer with the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Enhancer is important for boosting AAV production. If the enhancer is not used, it will reduce system performance by 50% or more.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the optimal incubation time for the plasmid DNA/AAV-MAX Transfection Reagent/AAV-MAX Transfection Booster complex?

We recommend a complexation time of 20-30 mins. We recommend optimizing the complexation time for different sized plasmids.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is it necessary to use the AAV-MAX Transfection Booster with the AAV-MAX Helper-Free AAV Production System? Will I see a drop in adeno-associated virus (AAV) titer if the booster is not used?

Yes, the AAV-MAX Transfection Booster facilitates transfection. If the booster is not used, it will reduce system performance.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.