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Pierce™ High Capacity Ni-IMAC Resin, EDTA compatible, 250 mL - FAQs

Pierce™ High Capacity Ni-IMAC Resin, EDTA compatible, 250 mL - FAQs

View additional product information for Pierce™ High-Capacity EDTA-Compatible Ni-IMAC Resin - FAQs (A40004565, A50587, A40004563, A50584, A50585, A50586)

7 product FAQs found

With Pierce High Capacity Ni-IMAC Resin, EDTA compatible, what residence times are adequate for FPLC purification?

Optimized residence times will differ by target, but the standard range is 2-10 mins.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How is Pierce High Capacity Ni-IMAC Resin, EDTA compatible different from standard Ni-NTA resin?

Pierce High Capacity Ni-IMAC Resin, EDTA compatible doesn't leach and discolor in the presence of reducing agent, EDTA, or cell culture supernatant, thereby providing exceptionally high protein yield.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

After regeneration of Pierce High Capacity Ni-IMAC Resin, EDTA compatible, is there a specific buffer the resin should be stored in?

The recommended storage buffer for the column and resin is 20% ethanol, 10 mM sodium acetate, pH 6.50. Please refer to the product manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0024862_PierceHighCapNiIMACResin_UG.pdf) for details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

With Pierce High Capacity Ni-IMAC Resin, EDTA compatible, how do I regenerate the resin?

Pierce High Capacity Ni-IMAC Resin, EDTA compatible is alkali-stable. The resin can be exposed to NaOH, washed with water, neutralized with PBS, washed with water, and put back into storage buffer. Please refer to the product manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0024862_PierceHighCapNiIMACResin_UG.pdf) for details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How many uses/regenerations are possible with Pierce High Capacity Ni-IMAC Resin, EDTA compatible?

Up to 10 uses are possible with Pierce High Capacity Ni-IMAC Resin, EDTA compatible.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What flow rates can I use with Pierce High Capacity Ni-IMAC Resin, EDTA compatible?

With Pierce High Capacity Ni-IMAC Resin, EDTA compatible, we recommend using a flow rate of 0.5-1.0 mL/min, but can go up to 2 mL/min depending on the sample type.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the binding capacity of Pierce High Capacity Ni-IMAC Resin, EDTA compatible?

The binding capacity of Pierce High Capacity Ni-IMAC Resin, EDTA compatible is up to 60 mg/mL.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.