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Qubit™ Protein BR Assay Kit, 500 assays - FAQs

Qubit™ Protein BR Assay Kit, 500 assays - FAQs

View additional product information for Qubit™ Protein and Protein Broad Range (BR) Assay Kits - FAQs (A50668, A50669, Q33212, Q33211)

7 product FAQs found

Is 1X RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40, 1mM EDTA, pH 7.4) compatible with the Qubit Protein Assay (Cat. No. Q33211/Q33212)?

The concentration of detergents in 1x RIPA buffer is quite high, therefore, we do not recommend that you use this kit with samples in RIPA. The Qubit Protein Assay has a tolerance for detergents present only in very low amounts. Please see the user guide for the tolerance of this assay for detergents here. The Qubit Protein Broad Range (BR) Assay Kit (A50668) has a much higher tolerance for detergents, e.g. RIPA buffer can be used undiluted. Please see this link.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

How can I use the Qubit Protein Broad Range Assay (Cat. No. A50668) on the Qubit 4 Fluorometer, with WiFi (Cat. No. Q33238)?

To be able to use the Qubit Protein Broad Range Assay (Cat. No. A50668) on the Qubit 4 Fluorometer (Cat. No Q33238) you would need to install the latest firmware on the fluorometer. Please find the latest Qubit 4 firmware (v2.19) on the following webpage: https://downloads.thermofisher.com/Qubit4/v2.19/Qubit4_update_v2.19.pak Save the firmware update on a USB stick and upload it to the Qubit 4 Fluorometer. Once the firmware update has been installed, you will be able to see the Protein BR assay on the Qubit 4 Fluorometer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Is the Quant-iT Protein Assay Kit (Cat. No. Q33210) a high-throughput version of Qubit Protein and Protein Broad Range Assay Kits?

Though the Quant-iT Protein Assay Kit is indicated for high-throughput application, it is completely separate from the Qubit Protein and Protein Broad Range Assay Kits, not another version of them.

Are the reagents in the Qubit Protein and Protein Broad Range Assay Kits and those from the Quant-iT Protein Assay Kit (Cat. No. Q33210) the same/interchangeable?

No, the Quant-it and Qubit reagents use different dyes and therefore are not interchangeable.

How do I get "Protein Broad Range" to appear in the Qubit 4 Fluorometer “Protein” menu required to read the standards and samples for the Qubit Protein BR Assay Kit (Cat. Nos. A50668, Q33211, Q33212, A50669)?

To access “Protein Broad Range” in the “Protein” menu of your Qubit 4 instrument, as instructed in the Qubit Protein BR Assay Kit (Cat. Nos. A50668, Q33211, Q33212, A50669) instructions, the instrument will require the latest firmware update (v2.19 or v1.8.0 for the Qubit Flex). You can check which version you currently have on your device by clicking on "Settings" from the Home screen, and then on "About Instrument".

To update the software:

Download the latest firmware update from the Qubit Fluorometers Technical Resources page.
Follow the instructions for updating your Qubit instrument in the firmware Release notes or in the Qubit 4 Fluorometer User Guide (page 53).

After the update has been completed, the option "Protein Broad Range" should become available.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Does the Qubit Protein Assay work well in the presence of detergents?

The Qubit Protein Assay is compatible with very small amounts of detergent. See “Contaminants Tolerated by the Qubit Protein Assay” Table 2 on page 6 of the Qubit Protein Assay Kit product manual (https://tools.thermofisher.com/content/sfs/manuals/Qubit_Protein_Assay_UG.pdf) for specific amounts.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am using the Qubit Protein and Protein Broad Range (BR) Assay Kits for quantifying protein concentration on a Qubit 4 Fluorometer. The kit contains 2 standards, however the Qubit 4 asks for a third standard. How can I work around this issue?

You will need to update your Qubit 4 Fluorometer with the latest firmware to be able to select the Qubit Protein BR assay. This assay is relatively new and is therefore not stored on the device. Please follow this link to download the latest firmware: https://www.thermofisher.com/de/de/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-technical-resources.html
In the event that this does not resolve the issue, please contact technical support at techsupport@thermofisher.com for further assistance.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.