The Thermo Scientific VitroEase Cryo-EM Training Kit provides a complete solution that includes detailed instructions for the successful preparation of grids for cryo-electron microscopy single particle analysis.
Features of the VitroEase Cryo-EM Training Kit include:
• Complete—contains an apoferritin positive-control sample, grids, accessories, and instructions for successful cryo-EM grid preparation
• Validated—apoferritin control protein production optimized and formulated specifically for successful use in cryo-EM grid sample preparation and analysis
• High-quality—grids and accessories from trusted sources
• Convenient—ready-to-use protein standard with step-by-step instructions for grid preparation
Sample preparation is a major bottleneck in cryo-electron microscopy (cryo-EM) single particle analysis (SPA). Currently, sample optimization is often performed in a non-systematic way, extending optimization time over multiple days or even weeks. The VitroEase Cryo-EM Training Kit has been designed to provide the necessary components to get started with cryo-EM analysis of protein samples.
The kit includes a positive control protein, apoferritin, that has been designed specifically to meet the stringent needs for high protein purity, minimal protein aggregation, and intact protein structure. The kit also contains commonly used accessories used in grid preparation, including tweezers for manipulation, grids, and grid boxes for storage. The detailed step-by-step instructions guide the user through the lengthy vitrification process. Together the control and components may be used as a comparison for success for new users or for quality control for expert users.