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Thermo Scientific™
SureQuant™ IP-MS Sample Preparation Kits
Thermo Scientific SureQuant IP-MS-Probenvorbereitungs-Kits bieten eine optimierte Lösung für die Immunpräzipitation und die Vorbereitung von MS-Peptiden von Proben vor der LC-MS/MS-Analyse.
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| Katalognummer | Beschreibung |
|---|---|
| A51744 | Streptavidin Magnetic Beads |
| A51743 | Protein AG Magnetic Beads |
Katalognummer A51744
Preis (EUR)
658,00
Each
Beschreibung:
Streptavidin Magnetic Beads
Preis (EUR)
658,00
Each
Thermo Scientific SureQuant IP-MS Probenvorbereitungs-Kits (Protein A/G oder Streptavidin) bieten eine optimierte Lösung für die Immunpräzipitation und die Vorbereitung von MS-Peptiden von Proben vor der LC-MS/MS-Analyse. Diese Kits wurden mit zahlreichen Multiplex-Zielantigenen mit unterschiedlichen Expressionsstufen sorgfältig verifiziert. Die SureQuant-Produktfamilie ermöglicht die Verwendung der Immunpräzipitation (IP)-Probenvorbereitungs-Kits und Peptid-Quantifizierungsmodule zusammen oder unabhängig für die SRM/PRM-Analyse.
• Complete—kits include reagents for successful immunoprecipitation and MS peptide preparation for target proteins for SRM/PRM analysis
• Multiplex—IP-MS kits have been designed for multiplex IP using both Protein A/G and Streptavidin magnetic beads
• Flexible—universal reagents enable individualized selection of targets for IP to be used alone or combined with SureQuant peptide quantitation panels
• Optimized—simplified and condensed procedure to prepare samples for LC-MS analysis
• Low background—binding, wash, and elution buffers optimized to remove contaminants and minimize enrichment of background proteins
The SureQuant Protein A/G and Streptavidin IP-MS Sample Preparation kits have been designed to support targeted quantitation using SureQuant or SRM/PRM analysis. The kits contain high-quality Pierce Protein A/G or Streptavidin magnetic beads together with reagents that have been optimized for MS compatibility. The streamlined procedure enables digestion immediately after the immunoprecipitation elution step, facilitating MS sample preparation in ∼4 hours for same-day LC-MS/MS analysis.
Applications:
• Enrichment of low abundance targets to improve MS sensitivity
• Identification of target proteins that are not detectable by western blotting
• Quantitation of target proteins by LC-MS analysis
• Screening of antibodies for IP-MS
• Enrichment of target proteins upstream of targeted analysis (SRM)
• Multiplex—IP-MS kits have been designed for multiplex IP using both Protein A/G and Streptavidin magnetic beads
• Flexible—universal reagents enable individualized selection of targets for IP to be used alone or combined with SureQuant peptide quantitation panels
• Optimized—simplified and condensed procedure to prepare samples for LC-MS analysis
• Low background—binding, wash, and elution buffers optimized to remove contaminants and minimize enrichment of background proteins
The SureQuant Protein A/G and Streptavidin IP-MS Sample Preparation kits have been designed to support targeted quantitation using SureQuant or SRM/PRM analysis. The kits contain high-quality Pierce Protein A/G or Streptavidin magnetic beads together with reagents that have been optimized for MS compatibility. The streamlined procedure enables digestion immediately after the immunoprecipitation elution step, facilitating MS sample preparation in ∼4 hours for same-day LC-MS/MS analysis.
Applications:
• Enrichment of low abundance targets to improve MS sensitivity
• Identification of target proteins that are not detectable by western blotting
• Quantitation of target proteins by LC-MS analysis
• Screening of antibodies for IP-MS
• Enrichment of target proteins upstream of targeted analysis (SRM)
Specifications
BeschreibungStreptavidin Magnetic Beads
Zur Verwendung mit (Anwendung)MS-kompatible Immunpräzipitation
Zur Verwendung mit (Geräte)Massenspektrometer
KitinhaltTrypsin-Protease, 2 x 20 µg
Trypsin-Lagerlösung, 250 µl
Streptavidin Magnetbeads, 1 ml
IP-MS-Zelllysepuffer, 100 ml
IP-MS Waschpuffer A, 75 ml
IP-MS Waschpuffer B, 40 ml
IP-Elution und MS-Probenvorbereitungspuffer, 10 ml
Alkylierungslösung, 1 ml/Fläschchen
Röhrchen mit niedriger Proteinbindeaufnahme, 50 x 1,5 ml
Bond-Breaker™ TCEP-Lösung, neutraler pH-Wert, 0,5 ml
10 % Pierce™ Trifluoressigsäure (TFA), Sequenzierungsgrad, 0,5 ml
Trypsin-Lagerlösung, 250 µl
Streptavidin Magnetbeads, 1 ml
IP-MS-Zelllysepuffer, 100 ml
IP-MS Waschpuffer A, 75 ml
IP-MS Waschpuffer B, 40 ml
IP-Elution und MS-Probenvorbereitungspuffer, 10 ml
Alkylierungslösung, 1 ml/Fläschchen
Röhrchen mit niedriger Proteinbindeaufnahme, 50 x 1,5 ml
Bond-Breaker™ TCEP-Lösung, neutraler pH-Wert, 0,5 ml
10 % Pierce™ Trifluoressigsäure (TFA), Sequenzierungsgrad, 0,5 ml
Menge20 Reaktionen
ArbeitsablaufschrittAnreicherung zur Probenvorbereitung
NachweisverfahrenMassenspektrometrie
ProduktlinieSureQuant
AusgangsmaterialZellen, Gewebe
Unit SizeEach
Inhalt und Lagerung
Trypsin bei 4 °C lagern.
Trypsin-Lagerungslösung bei -20 °C lagern.
Trypsin-Lagerungslösung bei -20 °C lagern.